Typical Testing Data/Standard Curve (for reference only)
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Canine Pancreatic amylase ELISA Kit | PAMY elisa kit
Canine pancreatic amylase (PAMY) ELISA Kit
Gene Names
Amy2a3; Amy2
Reactivity
Canine
Synonyms
Pancreatic amylase; Canine pancreatic amylase (PAMY) ELISA Kit; PAMY elisa kit
Reactivity
Canine
Sequence Length
508
Samples
Canine serum, plasma or cell culture supernatant
Assay Type
Quantitative Sandwich
Detection Range
20 ng/mL-0.312 ng/mL
Sensitivity
Up to 0.06 ng/mL
Intra-assay Precision
<= 8%
Inter-assay Precision
<= 12%
Preparation and Storage
Store all reagents at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
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Typical Testing Data/Standard Curve (for reference only)
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Related Product Information for PAMY elisa kit
Principle of the Assay: As mentioned above, this kit utilizes the Double Antibody Sandwich ELISA technique. The pre-coated antibody is an anti-Canine PAMY monoclonal antibody, while the detection antibody is a biotinylated polyclonal antibody. Samples and biotinylated antibodies are added into ELISA plate wells and washed out with PBS or TBS after their respective additions to the wells. Then Avidin-peroxidase conjugates are added to the wells in after. TMB substrate is used for coloration after the enzyme conjugate has already been thoroughly washed out of the wells by PBS or TBS. TMB reacts to form a blue product from the peroxidase activity, and finally turns to yellow after addition of the stop solution (Color Reagent C). The color intensity and quantity of target analyte in the sample are positively correlated.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
57,177 Da
NCBI Official Full Name
pancreatic alpha-amylase
NCBI Official Synonym Full Names
amylase 2a3
NCBI Official Symbol
Amy2a3
NCBI Official Synonym Symbols
Amy2
NCBI Protein Information
pancreatic alpha-amylase
UniProt Protein Name
Pancreatic alpha-amylase
UniProt Gene Name
Amy2
UniProt Synonym Gene Names
PA
UniProt Entry Name
AMYP_RAT
Uniprot Description
AMY2A: Amylases are secreted proteins that hydrolyze 1,4-alpha-glucoside bonds in oligosaccharides and polysaccharides, and thus catalyze the first step in digestion of dietary starch and glycogen. The human genome has a cluster of several amylase genes that are expressed at high levels in either salivary gland or pancreas. This gene encodes an amylase isoenzyme produced by the pancreas. [provided by RefSeq, Jul 2008]
Protein type: Carbohydrate Metabolism - starch and sucrose; Hydrolase; Secreted; Secreted, signal peptide; EC 3.2.1.1
Cellular Component: extracellular space
Molecular Function: alpha-amylase activity; calcium ion binding; cation binding; chloride ion binding; protein binding
Biological Process: carbohydrate catabolic process; carbohydrate metabolic process
Research Articles on PAMY
Similar Products
Product Notes
The Canine PAMY amy2 (Catalog #AAA2605183) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2605183 ELISA Kit recognizes Canine PAMY. It is sometimes possible for the material contained within the vial of "Pancreatic amylase, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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