Rabbit anti-Human Separase Polyclonal Antibody | anti-SEPA antibody

Separase, Phosphorylated (S1126) (ESPL1, ESP1, SEPA) (Biotin)

Cat. Num. AAA6436992

• Gene Names: ESPL1; ESP1; SEPA
• Reactivity: Human
• Applications: Immunofluorescence, Western Blot
• Purity: Purified by affinity chromatography.
• Synonyms: Separase; Polyclonal Antibody; Phosphorylated (S1126) (ESPL1; ESP1; SEPA) (Biotin); anti-SEPA antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human ESPL1, phosphorylated S1126.
• Purity/Purification: Purified by affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.
• Concentration: ~1mg/ml (varies by lot)

• Applicable Applications for anti-SEPA antibody: Immunofluorescence (IF), Western Blot (WB)
• Application Notes: IF: 1:20-1:100; WB: 1:500-1:2000; Applications are based on unconjugated antibody.
• Immunogen: Phospho specific peptide corresponding to residues surrounding S1126 of human ESPL1.
• Conjugate: Biotin
• Antigen Modification: Phosphorylated
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Immunofluorescence (IF)

(Immunofluorescence analysis of U2OS cell using Phospho-ESPL1-S1126 antibody. Blue: DAPI for nuclear staining.)

Product Categories/Family for anti-SEPA antibody

Antibodies; Apoptosis; Caspase

NCBI and Uniprot Product Information

• NCBI GI #: 134276943
• NCBI GeneID: 9700
• NCBI Accession #: NP_036423.4
• NCBI GenBank Nucleotide #: NM_012291.4
• UniProt Accession #: Q14674
• Molecular Weight: 233,175 Da
• NCBI Official Full Name: separin
• NCBI Official Synonym Full Names: extra spindle pole bodies homolog 1 (S. cerevisiae)
• NCBI Official Symbol: ESPL1
• NCBI Official Synonym Symbols: ESP1; SEPA
• NCBI Protein Information: separin; caspase-like protein ESPL1; extra spindle poles like 1; separin, cysteine protease; extra spindle poles-like 1 protein
• UniProt Protein Name: Separin
• Protein Family: Separase
• UniProt Gene Name: ESPL1
• UniProt Synonym Gene Names: ESP1; KIAA0165
• UniProt Entry Name: ESPL1_HUMAN

NCBI Description

Stable cohesion between sister chromatids before anaphase and their timely separation during anaphase are critical for chromosome inheritance. In vertebrates, sister chromatid cohesion is released in 2 steps via distinct mechanisms. The first step involves phosphorylation of STAG1 (MIM 604358) or STAG2 (MIM 300826) in the cohesin complex. The second step involves cleavage of the cohesin subunit SCC1 (RAD21; MIM 606462) by ESPL1, or separase, which initiates the final separation of sister chromatids (Sun et al., 2009 [PubMed 19345191]).[supplied by OMIM, Nov 2010]

Uniprot Description

separase: a caspase-like protease, which plays a central role in the chromosome segregation by cleaving the SCC1/RAD21 subunit of the cohesin complex at the onset of anaphase. During most of the cell cycle, it is inactivated by different mechanisms. Regulated by at least two independent mechanisms. First, it is inactivated via its interaction with securin/PTTG1, which probably covers its active site. Second, phosphorylation inactivates it. It is completely phosphorylated during mitosis and dephosphorylated during anaphase. Two alternatively spliced isoforms have been described.

Protein type: EC 3.4.22.49; Protease

Chromosomal Location of Human Ortholog: 12q

Cellular Component: centrosome; nucleus; cytosol

Molecular Function: protein binding; catalytic activity; cysteine-type peptidase activity

Biological Process: establishment of mitotic spindle localization; homologous chromosome segregation; mitotic sister chromatid segregation; apoptosis; negative regulation of sister chromatid cohesion; positive regulation of mitotic metaphase/anaphase transition; cytokinesis; mitotic cell cycle; proteolysis; meiotic spindle organization and biogenesis