Rabbit anti-Human, Mouse SENP2 Polyclonal Antibody | anti-SENP2 antibody

SENP2, CT (Sentrin SUMO-specific protease 2)

Cat. Num. AAA614534

• Gene Names: SENP2; AXAM2; SMT3IP2; KIAA1331; DKFZp762A2316
• Reactivity: Human, Mouse
• Applications: Western Blot
• Purity: Purified; Purified
• Synonyms: SENP2; Polyclonal Antibody; CT (Sentrin SUMO-specific protease 2); Anti -SENP2; anti-SENP2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Covalent attachment of one protein to another is one of the more prominent posttranslational modifications in respect to size and ubiquity. Ubiquitin is the most familiar of the protein modifiers and its activation and transfer to target proteins has been studied extensively. Recently, a new group of ubiquitin-like proteins has been receiving a lot of attention. SUMO, or Sentrin, is one of the most intriguing. SUMO has been implicated in the stabilization of target proteins and/or their localization to sub-cellular complexes.
• Purity/Purification: Purified; Purified
• Form/Format: Supplied as a liquid in PBS, 0.2% gelatin, 0.05% sodium azide.

• Applicable Applications for anti-SENP2 antibody: Western Blot (WB)
• Application Notes: Suitable for use in Western Blot.; Dilution: Western Blot: 1:500-1:1000; observed size: 60 kD; positive control: Jurkat or NIH3T3 whole cell lysate
• Immunogen: Synthetic peptide of human SENP2.
• Positive Control: Jurkat or NIH3T3 whole cell lysate
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Western Blot (WB)

(Western blot analysis of SENP2 in 15 ugs of Jurkat cell lysate using IMG-522 at 1:1000.)

Related Product Information for anti-SENP2 antibody

Covalent attachment of one protein to another is one of the more prominent posttranslational modifications in respect to size and ubiquity. Ubiquitin is the most familiar of the protein modifiers and its activation and transfer to target proteins has been studied extensively. Recently, a new group of ubiquitin-like proteins has been receiving a lot of attention. SUMO, or Sentrin, is one of the most intriguing. SUMO has been implicated in the stabilization of target proteins and/or their localization to sub-cellular complexes.

Conjugation of the small ubiquitin-like modifier SUMO-1/SMT3C/Sentrin-1 to proteins in vitro is dependent on a heterodimeric E1 (SAE1/SAE2) and an E2 (Ubc9). Although SUMO-2/SMT3A/Sentrin-3 and SUMO-3/SMT3B/Sentrin-2 share 50% sequence identity with SUMO-1, they are functionally distinct. Inspection of the SUMO-2 and SUMO-3 sequences indicates that they both contain the sequence psiKXE, which represents the consensus SUMO modification site. As a consequence SAE1/SAE2 and Ubc9 catalyze the formation of polymeric chains of SUMO-2 and SUMO-3 on protein substrates in vitro, and SUMO-2 chains are detected in vivo. The ability to form polymeric chains is not shared by SUMO-1, and although all SUMO species use the same conjugation machinery, modification by SUMO-1 and SUMO-2/-3 may have distinct functional consequences.

Product Categories/Family for anti-SENP2 antibody

Antibodies; Abs to Ubiquitin

NCBI and Uniprot Product Information

• NCBI GI #: 54607091
• NCBI GeneID: 59343
• NCBI Accession #: NP_067640.2
• NCBI GenBank Nucleotide #: NM_021627.2
• UniProt Accession #: Q9HC62; Q8IW97; Q96SR2; Q9P2L5
• Molecular Weight: 67,855 Da
• NCBI Official Full Name: sentrin-specific protease 2
• NCBI Official Synonym Full Names: SUMO1/sentrin/SMT3 specific peptidase 2
• NCBI Official Symbol: SENP2
• NCBI Official Synonym Symbols: AXAM2; SMT3IP2; KIAA1331; DKFZp762A2316
• NCBI Protein Information: sentrin-specific protease 2; OTTHUMP00000210348; SMT3-specific isopeptidase 2; sentrin/SUMO-specific protease SENP2; SUMO1/sentrin/SMT3 specific protease 2
• UniProt Protein Name: Sentrin-specific protease 2
• Protein Family: Sentrin-specific protease
• UniProt Gene Name: SENP2
• UniProt Synonym Gene Names: KIAA1331
• UniProt Entry Name: SENP2_HUMAN

NCBI Description

SUMO1 (UBL1; MIM 601912) is a small ubiquitin-like protein that can be covalently conjugated to other proteins. SENP2 is one of a group of enzymes that process newly synthesized SUMO1 into the conjugatable form and catalyze the deconjugation of SUMO1-containing species.[supplied by OMIM]

Uniprot Description

SENP2: Protease that catalyzes two essential functions in the SUMO pathway: processing of full-length SUMO1, SUMO2 and SUMO3 to their mature forms and deconjugation of SUMO1, SUMO2 and SUMO3 from targeted proteins. May down-regulate CTNNB1 levels and thereby modulate the Wnt pathway. Belongs to the peptidase C48 family.

Protein type: EC 3.4.22.68; Protease

Chromosomal Location of Human Ortholog: 3q27.2

Cellular Component: nucleoplasm; PML body; nuclear membrane; nuclear pore; cytoplasmic vesicle

Molecular Function: protein domain specific binding; protein binding; SUMO-specific protease activity

Biological Process: protein desumoylation; Wnt receptor signaling pathway; mRNA transport; heart development; regulation of Wnt receptor signaling pathway; dorsal/ventral axis specification; proteolysis; post-translational protein modification; regulation of DNA endoreduplication; negative regulation of DNA damage response, signal transduction by p53 class mediator; protein transport; protein sumoylation; cellular protein metabolic process; positive regulation of transcription from RNA polymerase II promoter; positive regulation of protein amino acid phosphorylation; negative regulation of protein binding