Immunohistochemistry (IHC)
((4ug/ml) staining of paraffin embedded Human Prostate. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.)
Goat Semaphorin 3E Polyclonal Antibody | anti-SEMA3E antibody
Goat anti-Semaphorin 3E (internal) Antibody
Immunohistochemistry: In paraffin embedded Human Prostate shows membranous staining of secretory cells in the gland. Recommended concentration, 4-6ug/ml.
Western Blot: Preliminary experiments gave an approx 35kDa band in Human Prostate lysates after 0. 03ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 89.2kDa according to NP_036563.1. The 35kDa band was successfully blocked by incubation with the immunizing peptide.
Immunohistochemistry (IHC)
((4ug/ml) staining of paraffin embedded Human Prostate. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.)
Immunohistochemistry (IHC)
((4ug/ml) staining of paraffin embedded Human Prostate. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.)
NCBI and Uniprot Product Information
NCBI Description
Semaphorins are a large family of conserved secreted and membrane associated proteins which possess a semaphorin (Sema) domain and a PSI domain (found in plexins, semaphorins and integrins) in the N-terminal extracellular portion. Based on sequence and structural similarities, semaphorins are put into eight classes: invertebrates contain classes 1 and 2, viruses have class V, and vertebrates contain classes 3-7. Semaphorins serve as axon guidance ligands via multimeric receptor complexes, some (if not all) containing plexin proteins. This gene encodes a class 4 semaphorin. This gene encodes a class 3 semaphorin. Multiple transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, May 2010]
Uniprot Description
SEMA3E: a secreted protein of the semaphorin family.
Protein type: Secreted, signal peptide; Secreted
Chromosomal Location of Human Ortholog: 7q21.11
Cellular Component: extracellular region; extracellular space
Molecular Function: chemorepellent activity; protein binding; semaphorin receptor binding
Biological Process: negative chemotaxis; negative regulation of angiogenesis; negative regulation of axon extension involved in axon guidance; negative regulation of cell-matrix adhesion; neural crest cell migration; patterning of blood vessels; positive regulation of cell migration; regulation of cell shape; sprouting angiogenesis; synapse organization and biogenesis
Disease: Charge Syndrome
Research Articles on SEMA3E
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Product Notes
The SEMA3E sema3e (Catalog #AAA420585) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-Semaphorin 3E (internal) Antibody reacts with Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig and may cross-react with other species as described in the data sheet. AAA Biotech's Semaphorin 3E can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Immunohistochemistry (IHC). Peptide ELISA: Antibody detection limit dilution 1: 128000. Immunohistochemistry: In paraffin embedded Human Prostate shows membranous staining of secretory cells in the gland. Recommended concentration, 4-6ug/ml. Western Blot: Preliminary experiments gave an approx 35kDa band in Human Prostate lysates after 0. 03ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 89.2kDa according to NP_036563.1. The 35kDa band was successfully blocked by incubation with the immunizing peptide. Researchers should empirically determine the suitability of the SEMA3E sema3e for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: NGGRYGTTKD YPDD. It is sometimes possible for the material contained within the vial of "Semaphorin 3E, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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