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Typical Testing Data/Standard Curve (for reference only)

Human Semaphorin 3E (SEMA3E) RTU ELISA Kit | SEMA3E rtu elisa kit

Human Semaphorin 3E (SEMA3E) ELISA Kit

Gene Names
SEMA3E; SEMAH; coll-5; M-SEMAH; M-SemaK
Reactivity
Human
Synonyms
Semaphorin 3E (SEMA3E); Human Semaphorin 3E (SEMA3E) ELISA Kit; SEMAH; M-SemaK; M-sema H; coll-5; Sema Domain; Immunoglobulin Domain(Ig); Transmembrane Domain(TM)and Short Cytoplasmic Domain 3E; SEMA3E rtu elisa kit
Ordering
For Research Use Only!
Reactivity
Human
Sequence Length
715
Assay Type
Sandwich
Samples
Serum, Plasma, Tissue Homogenates, Cell Lysates or Other Biological Fluids.
Detection Range
0.312-20ng/mL
Sensitivity
0.116ng/mL
RTU ELISA Kit's Advantage
The Ready-To-Use (RTU) Kits require fewer steps in setup and take less time to run. Detection Solutions are pre-diluted and total incubation time is shorter than many other ELISA Kits.
Preparation and Storage
For unopened kits: All the reagents should be kept at 4 degree C upon receipt.
For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.

Typical Testing Data/Standard Curve (for reference only)

Typical Testing Data/Standard Curve (for reference only)
Related Product Information for SEMA3E rtu elisa kit
Test Principle: The microtiter plate provided in this kit has been pre-coated with an antibody specific to SEMA3E. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to SEMA3E. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain SEMA3E, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of SEMA3E in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
82,424 Da
NCBI Official Full Name
semaphorin-3E isoform 2
NCBI Official Synonym Full Names
semaphorin 3E
NCBI Official Symbol
SEMA3E
NCBI Official Synonym Symbols
SEMAH; coll-5; M-SEMAH; M-SemaK
NCBI Protein Information
semaphorin-3E
UniProt Protein Name
Semaphorin-3E
Protein Family
UniProt Gene Name
SEMA3E
UniProt Synonym Gene Names
KIAA0331; SEMAH

NCBI Description

Semaphorins are a large family of conserved secreted and membrane associated proteins which possess a semaphorin (Sema) domain and a PSI domain (found in plexins, semaphorins and integrins) in the N-terminal extracellular portion. Based on sequence and structural similarities, semaphorins are put into eight classes: invertebrates contain classes 1 and 2, viruses have class V, and vertebrates contain classes 3-7. Semaphorins serve as axon guidance ligands via multimeric receptor complexes, some (if not all) containing plexin proteins. This gene encodes a class 4 semaphorin. This gene encodes a class 3 semaphorin. Multiple transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, May 2010]

Uniprot Description

Plays an important role in signaling via the cell surface receptor PLXND1. Mediates reorganization of the actin cytoskeleton, leading to the retraction of cell projections. Promotes focal adhesion disassembly and inhibits adhesion of endothelial cells to the extracellular matrix. Regulates angiogenesis, both during embryogenesis and after birth. Can down-regulate sprouting angiogenesis. Required for normal vascular patterning during embryogenesis. Plays an important role in ensuring the specificity of synapse formation ().

Research Articles on SEMA3E

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Product Notes

The Human SEMA3E sema3e (Catalog #AAA4502430) is a RTU ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA4502430 RTU ELISA Kit recognizes Human SEMA3E. It is sometimes possible for the material contained within the vial of "Semaphorin 3E (SEMA3E), RTU ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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