Rabbit SEMA3E Polyclonal Antibody | anti-SEMA3E antibody

SEMA3E Polyclonal Antibody

Cat. Num. AAA3006792

• Gene Names: SEMA3E; SEMAH; coll-5; M-SEMAH; M-SemaK
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: SEMA3E; Polyclonal Antibody; SEMA3E Polyclonal Antibody; Semaphorin-3E; Semaphorin 3E; KIAA0331; SEMAH; anti-SEMA3E antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: SEMA3E polyclonal antibody detects endogenous levels of SEMA3E protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 715

• Applicable Applications for anti-SEMA3E antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:1000
• Immunogen: A synthetic peptide corresponding to residues in Human SEMA3E.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of SEMA3E polyclonal antibody at 1:500 dilutionLane1:A549 cell lysateLane2:Mouse liver tissue lysateLane3:Rat liver tissue lysate)

Related Product Information for anti-SEMA3E antibody

Semaphorins are a family of cell surface and secreted proteins involved in neural development that are conserved from insects to humans. Members of this family are approximately 750 amino acids in length (including signal sequences) and are defined by a conserved extracellular "semaphorin" domain of approximately 500 amino acids containing 14-16 cysteines, blocks of conserved sequences and no obvious repeats. SEMA3E is a secreted semaphorin with 775 amino acids. Mutations in the SEMA3E gene are associated with CHARGE syndrome, a disorder characterized by cranial nerve dysfunction, coloboma of the eye, choanal atresia, inner and external ear abnormalities, cardiac anomalies, genitourinary abnormalities, and growth retardation.

NCBI and Uniprot Product Information

• NCBI GI #: 296011008
• NCBI GeneID: 9723
• NCBI Accession #: NP_001171600.1
• NCBI GenBank Nucleotide #: NP_001171600.1
• UniProt Accession #: O15041
• Molecular Weight: ~ 89kDa
• NCBI Official Full Name: semaphorin-3E isoform 2
• NCBI Official Synonym Full Names: semaphorin 3E
• NCBI Official Symbol: SEMA3E
• NCBI Official Synonym Symbols: SEMAH; coll-5; M-SEMAH; M-SemaK
• NCBI Protein Information: semaphorin-3E
• UniProt Protein Name: Semaphorin-3E
• Protein Family: Semaphorin
• UniProt Gene Name: SEMA3E
• UniProt Synonym Gene Names: KIAA0331; SEMAH

NCBI Description

Semaphorins are a large family of conserved secreted and membrane associated proteins which possess a semaphorin (Sema) domain and a PSI domain (found in plexins, semaphorins and integrins) in the N-terminal extracellular portion. Based on sequence and structural similarities, semaphorins are put into eight classes: invertebrates contain classes 1 and 2, viruses have class V, and vertebrates contain classes 3-7. Semaphorins serve as axon guidance ligands via multimeric receptor complexes, some (if not all) containing plexin proteins. This gene encodes a class 4 semaphorin. This gene encodes a class 3 semaphorin. Multiple transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, May 2010]

Uniprot Description

Plays an important role in signaling via the cell surface receptor PLXND1. Mediates reorganization of the actin cytoskeleton, leading to the retraction of cell projections. Promotes focal adhesion disassembly and inhibits adhesion of endothelial cells to the extracellular matrix. Regulates angiogenesis, both during embryogenesis and after birth. Can down-regulate sprouting angiogenesis. Required for normal vascular patterning during embryogenesis. Plays an important role in ensuring the specificity of synapse formation ().