Rabbit anti-Human SAR1A Polyclonal Antibody | anti-SAR1A antibody

SAR1A, ID (GTP-binding Protein SAR1a, COPII-associated Small GTPase, SARA1, SARA, SAR1) (Azide free) (HRP)

Cat. Num. AAA6498464

• Gene Names: SAR1A; SAR1; Sara; SARA1; masra2
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: SAR1A; Polyclonal Antibody; ID (GTP-binding Protein SAR1a; COPII-associated Small GTPase; SARA1; SARA; SAR1) (Azide free) (HRP); anti-SAR1A antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human SAR1A.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-SAR1A antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: ELISA: 1:1,000; WB: 1:100-1:500; IHC: 1:50-1:100; Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 122-149 in the central region of human SAR1A, UniProt Accession #Q9NR31. Species sequence homology: bovine, mouse and porcine
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis of MBS624191 in 293 (lane 1) and mouse lung tissue (lane 2) lysates (35ug/lane). SAR1A (arrow) was detected using the purified Pab.)

Western Blot (WB)

(Western Blot analysis of SAR1A (arrow) using MBS624191. 293 cell lysates (2ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the SAR1A gene.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human lung carcinoma withMBS624191, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.)

Flow Cytometry (FC/FACS)

(Flow Cytometric analysis of NCI-H292 cells using MBS624191 (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Product Categories/Family for anti-SAR1A antibody

Antibodies; Transport Proteins

References

1. Morgan, A.R., et.al., Am. J. Med. Genet. B Neuropsychiatr. Genet. 144B(6), 762-770(2007).

NCBI and Uniprot Product Information

• NCBI GI #: 9910542
• NCBI GeneID: 56681
• NCBI Accession #: NP_064535.1
• NCBI GenBank Nucleotide #: NM_020150.4
• UniProt Accession #: Q9NR31; B4DQ19
• Molecular Weight: 17,500 Da
• NCBI Official Full Name: GTP-binding protein SAR1a
• NCBI Official Synonym Full Names: secretion associated, Ras related GTPase 1A
• NCBI Official Symbol: SAR1A
• NCBI Official Synonym Symbols: SAR1; Sara; SARA1; masra2
• NCBI Protein Information: GTP-binding protein SAR1a; SAR1 homolog A; SAR1 gene homolog A; SAR1a gene homolog 1; COPII-associated small GTPase
• UniProt Protein Name: GTP-binding protein SAR1a
• Protein Family: GTP-binding protein
• UniProt Gene Name: SAR1A
• UniProt Synonym Gene Names: SAR1; SARA; SARA1
• UniProt Entry Name: SAR1A_HUMAN

Uniprot Description

SAR1A: Involved in transport from the endoplasmic reticulum to the Golgi apparatus. Required to maintain SEC16A localization at discrete locations on the ER membrane perhaps by preventing its dissociation. SAR1A-GTP-dependent assembly of SEC16A on the ER membrane forms an organized scaffold defining endoplasmic reticulum exit sites (ERES). Belongs to the small GTPase superfamily. SAR1 family.

Protein type: G protein, monomeric; G protein, monomeric, SAR1; G protein

Chromosomal Location of Human Ortholog: 10q22.1

Cellular Component: Golgi apparatus; sarcoplasmic reticulum

Molecular Function: GTP binding

Biological Process: vesicle-mediated transport; intracellular protein transport