Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat DNA damage-inducible transcript 4-like protein (DDIT4L) Standard Curve.)
Rat DNA Damage-Inducible Transcript 4-Like Protein (DDIT4L) ELISA Kit | DDIT4L elisa kit
Rat DNA Damage-Inducible Transcript 4-Like Protein (DDIT4L) ELISA Kit
Reactivity
Rat
Synonyms
DNA Damage-Inducible Transcript 4-Like Protein (DDIT4L); Rat DNA Damage-Inducible Transcript 4-Like Protein (DDIT4L) ELISA Kit; DDIT4L; REDD2; Rtp801L; homolog of mouse SMHS1; regulated in development and DNA damage response 2; DDIT4L elisa kit
Reactivity
Rat
Specificity
Rat DNA damage-inducible transcript 4-like protein (DDIT4L) ELISA Kit has high sensitivity and excellent specificity for detection of Rat DDIT4L. No significant cross-reactivity or interference between Rat DDIT4L and analogues was observed.
Sequence Length
193
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat DNA damage-inducible transcript 4-like protein (DDIT4L) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat DNA damage-inducible transcript 4-like protein (DDIT4L) Standard Curve.)
Related Product Information for DDIT4L elisa kit
Description: This Rat DNA damage-inducible transcript 4-like protein (DDIT4L) ELISA Kit employs a two-site sandwich ELISA to quantitate DDIT4L in samples. An antibody specific for DDIT4L has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any DDIT4L present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DDIT4L is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DDIT4L bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Inhibits cell growth by regulating the TOR signaling pathway upstream of the TSC1-TSC2 complex and downstream of AKT1.By similarity
Background: Inhibits cell growth by regulating the TOR signaling pathway upstream of the TSC1-TSC2 complex and downstream of AKT1.By similarity
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
DNA damage-inducible transcript 4-like protein
NCBI Official Synonym Full Names
DNA-damage-inducible transcript 4-like
NCBI Official Symbol
Ddit4l
NCBI Protein Information
DNA damage-inducible transcript 4-like protein
UniProt Protein Name
DNA damage-inducible transcript 4-like protein
Protein Family
UniProt Gene Name
Ddit4l
UniProt Synonym Gene Names
Smhs1
Uniprot Description
DDIT4L: Inhibits cell growth by regulating the TOR signaling pathway upstream of the TSC1-TSC2 complex and downstream of AKT1. Belongs to the DDIT4 family.
Protein type: Unknown function
Chromosomal Location of Human Ortholog: 3q21
Cellular Component: cytoplasm
Biological Process: negative regulation of signal transduction
Similar Products
Product Notes
The Rat DDIT4L ddit4l (Catalog #AAA9712459) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9712459 ELISA Kit recognizes Rat DDIT4L. It is sometimes possible for the material contained within the vial of "DNA Damage-Inducible Transcript 4-Like Protein (DDIT4L), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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