PINK1 recombinant protein

PINK1, 156-507aa, Human, E Coli (Denatured)

Cat. Num. AAA204731

• Gene Names: PINK1; BRPK; PARK6
• Applications: SDS-Page
• Purity: > 90% by SDS-PAGE
• Synonyms: PINK1; 156-507aa; Human; E Coli (Denatured); Serine/threonine-protein kinase PINK1 mitochondrial; Serine/threonine-protein kinase PINK1; mitochondrial; BRPK; FLJ27236; PARK6; PINK1 recombinant protein

• Host: E Coli
• Purity/Purification: > 90% by SDS-PAGE
• Form/Format: Liquid. In 20mM Tris-HCl Buffer (pH 8.0) 1M urea, 5% glycerol.
• Concentration: 1 mg/ml (determined by Bradford assay) (varies by lot)
• Sequence: MYLIGQSIGK GCSAAVYEAT MPTLPQNLEV TKSTGLLPGR GPGTSAPGEG QERAPGAPAF PLAIKMMWNI SAGSSSEAIL NTMSQELVPA SRVALAGEYG AVTYRKSKRG PKQLAPHPNI IRVLRAFTSS VPLLPGALVD YPDVLPSRLH PEGLGHGRTL FLVMKNYPCT LRQYLCVNTP SPRLAAMMLL QLLEGVDHLV QQGIAHRDLK SDNILVELDP DGCPWLVIAD FGCCLADESI GLQLPFSSWY VDRGGNGCLM APEVSTARPG PRAVIDYSKA DAWAVGAIAY EIFGLVNPFY GQGKAHLESR SYQEAQLPAL PESVPPDVRQ LVRALLQREA SKRPSARVAA NVL
• Sequence Length: 581

• Applicable Applications for PINK1 recombinant protein: SDS-PAGE
• Antigen Species: Human
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for PINK1 recombinant protein

PINK1 is a serine/threonine protein kinase that localizes to mitochondria. It is thought to protect cells from stress-induced mitochondrial dysfunction. Mutations in this protein cause one form of autosomal recessive early-onset Parkinson disease. Recombinant human PINK protein was expressed in E Coli and purified by using conventional chromatography techniques.

Product Categories/Family for PINK1 recombinant protein

Neuroscience

References

Valente EM., et al. (2004) Ann Neurol. 56 (3):336-41.; unoki M., et al. (2001) Oncogene. 20 (33):4457-65.

NCBI and Uniprot Product Information

• NCBI GI #: 20381329
• NCBI GeneID: 65018
• Molecular Weight: 37.9 kDa (353aa)
• NCBI Official Full Name: PTEN induced putative kinase 1
• NCBI Official Synonym Full Names: PTEN induced putative kinase 1
• NCBI Official Symbol: PINK1
• NCBI Official Synonym Symbols: BRPK; PARK6
• NCBI Protein Information: serine/threonine-protein kinase PINK1, mitochondrial
• UniProt Protein Name: Serine/threonine-protein kinase PINK1, mitochondrial
• Protein Family: Serine/threonine-protein kinase
• UniProt Gene Name: PINK1

NCBI Description

This gene encodes a serine/threonine protein kinase that localizes to mitochondria. It is thought to protect cells from stress-induced mitochondrial dysfunction. Mutations in this gene cause one form of autosomal recessive early-onset Parkinson disease. [provided by RefSeq, Jul 2008]

Uniprot Description

Protects against mitochondrial dysfunction during cellular stress by phosphorylating mitochondrial proteins. Involved in the clearance of damaged mitochondria via selective autophagy (mitophagy) by mediating activation and translocation of PRKN (PubMed:14607334, PubMed:15087508, PubMed:19229105, PubMed:19966284, PubMed:20404107, PubMed:20798600, PubMed:23620051, PubMed:23754282, PubMed:23933751, PubMed:24660806, PubMed:24751536, PubMed:24784582, PubMed:24896179, PubMed:25527291). Targets PRKN to dysfunctional depolarized mitochondria through the phosphorylation of MFN2 (PubMed:23620051). Activates PRKN in 2 steps: (1) by mediating phosphorylation at 'Ser-65' of PRKN and (2) mediating phosphorylation of ubiquitin, converting PRKN to its fully-active form (PubMed:24660806, PubMed:24751536, PubMed:24784582, PubMed:25527291). Required for ubiquinone reduction by mitochondrial complex I by mediating phosphorylation of complex I subunit NDUFA10 ().