Rabbit Sam 68 Polyclonal Antibody | anti-Sam68 antibody

Sam 68 Polyclonal Antibody

Cat. Num. AAA3008699

• Gene Names: KHDRBS1; p62; p68; Sam68
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: Sam 68; Polyclonal Antibody; Sam 68 Polyclonal Antibody; p62; p68; Sam68; anti-Sam68 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Sam 68 polyclonal antibody detects endogenous levels of Sam 68 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 443

• Applicable Applications for anti-Sam68 antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: WB: 1:500-1:2000; IHC: 1:100-1:200; IF: 1:100-1:200
• Immunogen: A synthetic peptide of human KHDRBS1.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot analysis of extracts of various cell lines, using KHDRBS1 antibody.)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded rat brain using KHDRBS1 antibody at dilution of 1:100 (40x lens).)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human prostate cancer using KHDRBS1 antibody at dilution of 1:100 (40x lens).)

Related Product Information for anti-Sam68 antibody

SAM68 is recruited and tyrosine phosphorylated by several receptor systems, and once it is phosphorylated, it functions as an adapter protein in signal transduction cascades by binding to SH2 and SH3 domain-containing proteins.It represses CBP-dependent transcriptional activation apparently by competing with other nuclear factors for binding to CBP. It also acts as a putative regulator of mRNA stability and/or translation rates and mediates mRNA nuclear export.

NCBI and Uniprot Product Information

• NCBI GI #: 62511098
• NCBI GeneID: 10657
• UniProt Accession #: Q07666
• Molecular Weight: ~ 68kDa
• NCBI Official Full Name: KH domain-containing, RNA-binding, signal transduction-associated protein 1
• NCBI Official Synonym Full Names: KH RNA binding domain containing, signal transduction associated 1
• NCBI Official Symbol: KHDRBS1
• NCBI Official Synonym Symbols: p62; p68; Sam68
• NCBI Protein Information: KH domain-containing, RNA-binding, signal transduction-associated protein 1
• UniProt Protein Name: KH domain-containing, RNA-binding, signal transduction-associated protein 1
• UniProt Gene Name: KHDRBS1
• UniProt Synonym Gene Names: Sam68
• UniProt Entry Name: KHDR1_HUMAN

NCBI Description

This gene encodes a member of the K homology domain-containing, RNA-binding, signal transduction-associated protein family. The encoded protein appears to have many functions and may be involved in a variety of cellular processes, including alternative splicing, cell cycle regulation, RNA 3'-end formation, tumorigenesis, and regulation of human immunodeficiency virus gene expression. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2012]

Uniprot Description

KHDRBS1: a nuclear RNA-binding protein. May be involved in slicing and mRNA transport. Associates with src in mitosis. Binds to RNA via a heteronuclear ribonucleoprotein K homology domain. Exerts functionally separable effects on cell proliferation and survival, dependent on its ability to bind specifically to RNA.

Protein type: RNA-binding

Chromosomal Location of Human Ortholog: 1p32

Cellular Component: nucleoplasm; membrane; cytoplasm; nucleus

Molecular Function: identical protein binding; protein binding; SH3/SH2 adaptor activity; DNA binding; RNA binding; protein complex binding; poly(U) binding; SH3 domain binding; poly(A) binding

Biological Process: transcription, DNA-dependent; positive regulation of signal transduction; positive regulation of RNA export from nucleus; regulation of protein stability; signal transduction; positive regulation of translational initiation; cell proliferation; cell surface receptor linked signal transduction; regulation of RNA export from nucleus; mRNA processing; cell cycle arrest; G2/M transition of mitotic cell cycle; negative regulation of transcription, DNA-dependent