Typical Testing Data/Standard Curve (for reference only)
Human CDK1 (Cyclin Dependent Kinase 1) CLIA Kit | CDK1 clia kit
Human CDK1 (Cyclin Dependent Kinase 1) CLIA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: This CLIA kit usesthe Competitive-CLIAprinciple. The micro CLIAplate provided in this kit has been pre-coated with Human 8-epi-PGF2alpha. During the reaction. Human 8-epi-PGF2alpha in the sample or standard competes with a fixed amount of Human8-epi-PGF2alphaon the solid phase supporter for sites on the Biotinylated Detection Ab specific to Human 8-epi-PGF2alpha. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The Relative light unit (RLU) is measured spectrophotometricallyby the Chemiluminescence immunoassay analyzer. The RLU value is negatively associated with the concentration of Human 8-epi-PGF2alpha. You can calculate the concentration of Human 8-epi-PGF2alpha in the samples by comparing the RLU of the samples to the standard curve.
NCBI and Uniprot Product Information
Uniprot Description
CDK1: a protein kinase of the CDK family. Catalytic subunit of the conserved protein complex known as M-phase promoting factor (MPF), which is essential for G1/S and G2/M phase transitions. Mitotic cyclins stably associate with this protein and function as regulatory subunits. Its activity is controlled by cyclin availability and phosphorylation through the cell cycle. Activated in many cancers including colon, liver and breast. The T isoform, which lacks a regulatory region, is expressed in breast cancer. Inhibition in cancer cells may drive cells into apoptosis. May also drive cell migration. Inhibitors: BMS-265246, BMS-265246-01. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: EC 2.7.11.22; Protein kinase, CMGC; Cell cycle regulation; Protein kinase, Ser/Thr (non-receptor); Motility/polarity/chemotaxis; EC 2.7.11.23; Kinase, protein; CMGC group; CDK family; CDK/CDK1 subfamily; CDK1 subfamily
Cellular Component: centrosome; cytoskeleton; membrane; mitochondrion; spindle microtubule; cytoplasm; midbody; nucleus
Molecular Function: transferase activity; RNA polymerase subunit kinase activity; protein serine/threonine kinase activity; cyclin binding; protein binding; cyclin-dependent protein kinase activity; nucleotide binding; histone kinase activity; kinase activity; Hsp70 protein binding; ATP binding; protein kinase activity
Biological Process: mitosis; organ regeneration; positive regulation of protein import into nucleus, translocation; apoptosis; histone phosphorylation; peptidyl-threonine phosphorylation; cell aging; response to organic nitrogen; positive regulation of mitotic cell cycle; cell cycle; protein amino acid phosphorylation; chromosome condensation; peptidyl-serine phosphorylation; response to ethanol; cell division; mitotic cell cycle G2/M transition DNA damage checkpoint; protein complex assembly; phosphorylation; positive regulation of DNA replication; positive regulation of cardiac muscle cell proliferation; negative regulation of apoptosis
Research Articles on CDK1
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Product Notes
The Human CDK1 cdk1 (Catalog #AAA2531151) is a CLIA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2531151 CLIA Kit recognizes Human CDK1. It is sometimes possible for the material contained within the vial of "CDK1 (Cyclin Dependent Kinase 1), CLIA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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