Typical Testing Data/Standard Curve (for reference only)
Mouse Synaptophysin ELISA Kit | SYP elisa kit
Mouse Synaptophysin ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: SYP ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-SYP antibody and an SYP-HRP conjugate. The assay sample and buffer are incubated together with SYP-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the SYP concentration since SYP from samples and SYP-HRP conjugate compete for the anti-SYP antibody binding site. Since the number of sites is limited, as more sites are occupied by SYP from the sample, fewer sites are left to bind SYP-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The SYP concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes an integral membrane protein of small synaptic vesicles in brain and endocrine cells. The protein also binds cholesterol and is thought to direct targeting of vesicle-associated membrane protein 2 (synaptobrevin) to intracellular compartments. Mutations in this gene are associated with X-linked mental retardation (XLMR). [provided by RefSeq, Aug 2011]
Uniprot Description
SYP: Possibly involved in structural functions as organizing other membrane components or in targeting the vesicles to the plasma membrane. Involved in the regulation of short-term and long-term synaptic plasticity. Homohexamer or homotetramer. Interacts with SRCIN1. Characteristic of a type of small (30-80 nm) neurosecretory vesicles, including presynaptic vesicles, but also vesicles of various neuroendocrine cells of both neuronal and epithelial phenotype. Belongs to the synaptophysin/synaptobrevin family.
Protein type: Vesicle; Membrane protein, integral; Membrane protein, multi-pass
Chromosomal Location of Human Ortholog: Xp11.23-p11.22
Cellular Component: presynaptic membrane; synaptic vesicle; neuron projection; protein complex; synaptic vesicle membrane; presynaptic active zone; excitatory synapse; cell junction; integral to synaptic vesicle membrane
Molecular Function: identical protein binding; protein self-association; transporter activity; protein complex binding; syntaxin-1 binding; cholesterol binding; SH2 domain binding
Biological Process: regulation of long-term neuronal synaptic plasticity; synaptic vesicle maturation; synaptic vesicle membrane organization and biogenesis; endocytosis; regulation of short-term neuronal synaptic plasticity; regulation of neuronal synaptic plasticity
Disease: Mental Retardation, X-linked 96
Research Articles on SYP
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Product Notes
The Mouse SYP syp (Catalog #AAA721768) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA721768 ELISA Kit recognizes Mouse SYP. It is sometimes possible for the material contained within the vial of "Synaptophysin, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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