Rabbit anti-Human SAFB2 Polyclonal Antibody | anti-SAFB2 antibody

Anti-SAFB2 Antibody, Rabbit Polyclonal

Cat. Num. AAA8109697

• Reactivity: Human
• Applications: Immunohistochemistry, Immunocytochemistry, Immunofluorescence
• Purity: Protein A & Antigen Affinity
• Synonyms: SAFB2; Polyclonal Antibody; Anti-SAFB2 Antibody; Rabbit Polyclonal; SAFB2/Scaffold attachment factor B2 Antibody; Rabbit PAb; Antigen Affinity Purified; Anti-Scaffold attachment factor B2 Antibody; anti-SAFB2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human SAFB2/Scaffold attachment factor B2
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; PBS, pH7.0 with 0.03% Proclin300

• Applicable Applications for anti-SAFB2 antibody: Immunohistochemistry-Paraffin (IHC-P), Immunocytochemistry (ICC), Immunofluorescence (IF)
• Application Notes: IHC-P: 1:50-1:200; ICC/IF: 1:100-1:500
• Immunogen: E Coli-derived Human SAFB2/Scaffold attachment factor B2 fragment
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with E Coli-derived Human SAFB2/Scaffold attachment factor B2 fragment, and purified by antigen affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Avoid repeated freeze-thaw cycles.

Immunohistochemistry (IHC)

(Immunochemical staining of human SAFB2 in human kidney with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)

Immunohistochemistry (IHC)

(Immunochemical staining of human SAFB2 in human brain with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)

Immunofluorescence (IF)

(Immunofluorescence staining of SAFB2 in MCF7 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human SAFB2 polyclonal antibody (dilution ratio 1:200) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Nucleus.)

Related Product Information for anti-SAFB2 antibody

Scaffold attachment factor B1 (SAFB1) and SAFB2 are large, multifunctional proteins that have been implicated in numerous cellular processes including chromatin organization, transcriptional regulation, RNA splicing, and stress response. SAFB1 and SAFB2 function as estrogen receptor (ERalpha) co-repressors in breast cancer cells, and to affect many cellular processes such as stress response, RNA processing, and apoptosis. SAFB2 can be found in both the nucleus and cytoplasm, whereas SAFB1 seems to be mainly localized in the nucleus. In breast cancer cells, SAFBs function as estrogen receptor corepressors and growth inhibitors. SAFB1 and SAFB2 have also been implicated in breast tumorigenesis. SAFB protein expression is lost in approximately 20% of breast cancers. The two genes reside in close proximity, oriented head-to-head, on chromosome 19p13, a locus that is frequently lost in clinical breast cancer specimens.

References

Oesterreich S (2003) Scaffold attachment factors safb1 and safb2: Innocent bystanders or critical players in breast tumorigenesis? J Cell Biochem 90 (4): 653-661.Hong EA, et al. (2012) Safb1-and safb2-mediated transcriptional repression: Relevance to cancer. Biochem Soc Trans 40 (4): 826-830.Hammerich-Hille S, et al. (2010) Low safb levels are associated with worse outcome in breast cancer patients. Breast Cancer Res Treat 121 (2): 503-509.