Immunohistochemistry (IHC)
(Immunochemical staining of human RBFOX1 in human brain with rabbit polyclonal antibody at 1:2000 dilution, formalin-fixed paraffin embedded sections.)
Rabbit anti-Human RBFOX1 Polyclonal Antibody | anti-RBFOX1 antibody
Anti-RBFOX1 Antibody, Rabbit Polyclonal
Reactivity
Human
Applications
Immunohistochemistry, Immunocytochemistry, Immunofluorescence
Purity
Protein A & Antigen Affinity
Synonyms
RBFOX1; Polyclonal Antibody; Anti-RBFOX1 Antibody; Rabbit Polyclonal; RBFOX1 Antibody; Rabbit PAb; Antigen Affinity Purified; RNA binding protein fox-1 homolog 1; Anti-2BP1 Antibody; Anti-A2BP1 Antibody; Anti-FOX-1 Antibody; Anti-FOX1 Antibody; Anti-HRNBP1 Antibody; anti-RBFOX1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human RBFOX1
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; PBS, pH7.0 with 0.03% Proclin300
Applicable Applications for anti-RBFOX1 antibody
Immunohistochemistry-Paraffin (IHC-P), Immunocytochemistry (ICC), Immunofluorescence (IF)
Application Notes
IHC-P: 1:500-1:5000
ICC/IF: 1:100-1:500
ICC/IF: 1:100-1:500
Immunogen
E Coli-derived Human RBFOX1 fragment
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with E Coli-derived Human RBFOX1 fragment, and purified by antigen affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Avoid repeated freeze-thaw cycles.
Immunohistochemistry (IHC)
(Immunochemical staining of human RBFOX1 in human brain with rabbit polyclonal antibody at 1:2000 dilution, formalin-fixed paraffin embedded sections.)
Immunohistochemistry (IHC)
(Immunochemical staining of human RBFOX1 in human brain with rabbit polyclonal antibody at 1:2000 dilution, formalin-fixed paraffin embedded sections.)
Immunofluorescence (IF)
(Immunofluorescence staining of RBFOX1 in U2OS cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human RBFOX1 polyclonal antibody (dilution ratio 1:200) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Nucleus and Cytoplasm.)
Immunofluorescence (IF)
(Immunofluorescence staining of RBFOX1 in U2OS cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human RBFOX1 polyclonal antibody (dilution ratio 1:200) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Nucleus and Cytoplasm.)
Related Product Information for anti-RBFOX1 antibody
RBFOX1 (also known as FOX1 and A2BP1), an RNA splicing factor, regulates the expression of large genetic networks during early neuronal development, and haploinsufficiency causes severe neurodevelopmental phenotypes including autism spectrum disorder, mental retardation, epilepsy, attention-deficit/hyperactivity disorder, and schizophrenia. Human genetic studies have identified the neuronal RNA binding protein, Rbfox1, as a candidate gene for autism spectrum disorders. The RNA-binding protein RBFOX1 is an important regulator of neuron development and neuronal excitability. Rbfox1 is abundant in the vertebrate brain, heart, and skeletal muscle, and can regulate developmental and tissue-specific alternative splicing in heart, muscle, and neuronal tissues. The RBFOX1 protein and its homologs (RBFOX2 and RBFOX3) regulate alternative splicing of many neuronal transcripts involved in the homeostatic control of neuronal excitability.
References
Zhao WW (2013) Intragenic deletion of rbfox1 associated with neurodevelopmental/neuropsychiatric disorders and possibly other clinical presentations. Mol Cytogenet 6 (1): 26.Lee JA, et al. (2016) Cytoplasmic rbfox1 regulates the expression of synaptic and autism-related genes. Neuron 89 (1): 113-128.Gu L, et al. (2018) Downregulation of splicing regulator rbfox1 compromises visual depth perception. PLoS One 13 (7): e0200417.Bill BR, et al. (2013) Orchestration of neurodevelopmental programs by rbfox1: Implications for autism spectrum disorder. Int Rev Neurobiol 113 251-267.
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Product Notes
The RBFOX1 (Catalog #AAA8109707) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-RBFOX1 Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's RBFOX1 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry-Paraffin (IHC-P), Immunocytochemistry (ICC), Immunofluorescence (IF). IHC-P: 1:500-1:5000 ICC/IF: 1:100-1:500. Researchers should empirically determine the suitability of the RBFOX1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RBFOX1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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