Western Blot (WB)
(SACA1 Antibody (N-term) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the SACA1 antibody detected the SACA1 protein (arrow).)
Rabbit anti-Human SACA1 Polyclonal Antibody | anti-SPACA1 antibody
SACA1 Antibody (N-term)
Western Blot (WB)
(SACA1 Antibody (N-term) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the SACA1 antibody detected the SACA1 protein (arrow).)
Western Blot (WB)
(SACA1 Antibody (N-term) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the SACA1 antibody detected the SACA1 protein (arrow).)
Immunohistochemistry (IHC)
(SACA1 antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the SACA1 antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(SACA1 antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the SACA1 antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Flow Cytometry (FC/FACS)
(SACA1 Antibody (N-term) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Flow Cytometry (FC/FACS)
(SACA1 Antibody (N-term) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
unexplained infertility implicates a role for these antibodies in
blocking fertilization. Improved identification and treatment of
immunologic infertility, as well as identification of proteins for
targeted contraception, are dependent on the identification and
characterization of relevant sperm antigens. The protein expressed
by this gene is recognized by anti-sperm antibodies from infertile
males. Furthermore, antibodies generated against the recombinant
protein block in vitro fertilization. This protein localizes to the
acrosomal membrane of spermatids and mature spermatozoa where it is
thought to play a role in acrosomal morphogenesis and in sperm-egg
binding and fusion, respectively.
Levy, D., et al. BMC Med. Genet. 8 SUPPL 1, S3 (2007) :
Vasan, R.S., et al. BMC Med. Genet. 8 SUPPL 1, S2 (2007) :
Mungall, A.J., et al. Nature 425(6960):805-811(2003)
Hao, Z., et al. Biol. Reprod. 66(3):735-744(2002)
NCBI and Uniprot Product Information
NCBI Description
The correlation of anti-sperm antibodies with cases of unexplained infertility implicates a role for these antibodies in blocking fertilization. Improved diagnosis and treatment of immunologic infertility, as well as identification of proteins for targeted contraception, are dependent on the identification and characterization of relevant sperm antigens. The protein expressed by this gene is recognized by anti-sperm antibodies from infertile males. Furthermore, antibodies generated against the recombinant protein block in vitro fertilization. This protein localizes to the acrosomal membrane of spermatids and mature spermatozoa where it is thought to play a role in acrosomal morphogenesis and in sperm-egg binding and fusion, respectively. [provided by RefSeq, Jul 2008]
Uniprot Description
SPACA1: May be involved in sperm-egg fusion.
Protein type: Membrane protein, integral
Chromosomal Location of Human Ortholog: 6q15
Cellular Component: integral to membrane; acrosomal membrane
Biological Process: acrosome formation
Research Articles on SPACA1
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Product Notes
The SPACA1 spaca1 (Catalog #AAA9208412) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 46-74. The SACA1 Antibody (N-term) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's SACA1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS). WB~~1:1000. Researchers should empirically determine the suitability of the SPACA1 spaca1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SACA1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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