Western Blot (WB)
(Western Blot analysis of 293 cell line lysate (35ug/lane) using MBS6008413. This demonstrates that MBS6008413 detected the SACA1 protein (arrow).)
Rabbit anti-Human SACA1 Polyclonal Antibody | anti-sacA1 antibody
SACA1, NT (SPACA1, SAMP32, Sperm acrosome membrane-associated protein 1, Sperm acrosomal membrane-associated protein 32)
Reactivity
Human
Applications
ELISA, Western Blot, Immunohistochemistry, Flow Cytometry, Functional Assay
Purity
Affinity PurifiedPurified by Protein A affinity chromatography.
Synonyms
SACA1; Polyclonal Antibody; NT (SPACA1; SAMP32; Sperm acrosome membrane-associated protein 1; Sperm acrosomal membrane-associated protein 32); Anti -SACA1; anti-sacA1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Human
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Purified by Protein A affinity chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Applicable Applications for anti-sacA1 antibody
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
Application Notes
Suitable for use in Western Blot, Immunohistochemistry, Flow Cytometry, ELISA
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500
Immunohistochemistry: 1:50-100
Flow Cytometry: 1:10-50
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500
Immunohistochemistry: 1:50-100
Flow Cytometry: 1:10-50
Immunogen
SACA1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 49-78 amino acids from the N-terminal region of human SACA1.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(Western Blot analysis of 293 cell line lysate (35ug/lane) using MBS6008413. This demonstrates that MBS6008413 detected the SACA1 protein (arrow).)
Western Blot (WB)
(Western Blot analysis of 293 cell line lysate (35ug/lane) using MBS6008413. This demonstrates that MBS6008413 detected the SACA1 protein (arrow).)
Immunohistochemistry (IHC)
(Immunohistochemistry analysis of formalin fixed and paraffin embedded human testis tissue using MBS6008413. Peroxidase-conjugated secondary antibody and DAB were used for visualization.)
Immunohistochemistry (IHC)
(Immunohistochemistry analysis of formalin fixed and paraffin embedded human testis tissue using MBS6008413. Peroxidase-conjugated secondary antibody and DAB were used for visualization.)
Flow Cytometry (FC/FACS)
(Flow Cytometric analysis of 293 cells using MBS6008413 (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Flow Cytometry (FC/FACS)
(Flow Cytometric analysis of 293 cells using MBS6008413 (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Related Product Information for anti-sacA1 antibody
The correlation of anti-sperm antibodies with cases of unexplained infertility implicates a role for these antibodies in blocking fertilization. Improved identification and treatment of immunologic infertility, as well as identification of proteins for targeted contraception, are dependent on the identification and characterization of relevant sperm antigens. The protein expressed by this gene is recognized by anti-sperm antibodies from infertile males. Furthermore, antibodies generated against the recombinant protein block in vitro fertilization. This protein localizes to the acrosomal membrane of spermatids and mature spermatozoa where it is thought to play a role in acrosomal morphogenesis and in sperm-egg binding and fusion, respectively.
Product Categories/Family for anti-sacA1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
55,346 Da
NCBI Official Full Name
sucrose-6-phosphate hydrolase SacA
NCBI Official Symbol
sacA1
NCBI Protein Information
sucrose-6-phosphate hydrolase SacA
UniProt Protein Name
Glycoside Hydrolase Family 32
UniProt Gene Name
sacA1
UniProt Synonym Gene Names
sacA
UniProt Entry Name
Q65DN5_BACLD
Uniprot Description
Sequence similarities: Belongs to the glycosyl hydrolase 32 family. RuleBase RU000449
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Product Notes
The sacA1 saca1 (Catalog #AAA6008413) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The SACA1, NT (SPACA1, SAMP32, Sperm acrosome membrane-associated protein 1, Sperm acrosomal membrane-associated protein 32) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's SACA1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS). Suitable for use in Western Blot, Immunohistochemistry, Flow Cytometry, ELISA Dilution: ELISA: 1:1,000 Western Blot: 1:100-500 Immunohistochemistry: 1:50-100 Flow Cytometry: 1:10-50. Researchers should empirically determine the suitability of the sacA1 saca1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SACA1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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