Rabbit anti-Human S100A8 Polyclonal Antibody | anti-S100A8 antibody

S100A8 Polyclonal Antibody

Cat. Num. AAA2554121

• Reactivity: Human
• Applications: Immunohistochemistry
• Purity: Affinity Purification
• Synonyms: S100A8; Polyclonal Antibody; S100A8 Polyclonal Antibody; 60B8Ag; AI323541; B8Ag; BEE11; CAGA; Calgranulin-A; Calprotectin L1L subunit; Calprotectin; included; CFAG; CGLA; Chemotactic cytokine CP-10; CP-10; Cystic fibrosis antigen; L1Ag; Leukocyte L1 complex light chain; MA387; MIF; Migration inhibitory factor-related protein 8; MRP-8; Myeloid-related protein 8; Neutrophil cytosolic 7 kDa protein; NIF; p8; Pro-inflammatory S100 cytokine; Protein S100-A8; S100 calcium binding protein A8 (calgranulin A); S100 calcium binding protein A8; S100 calcium-binding protein A8; S100A8/S100A9 complex; S10A8; Urinary stone protein band A; anti-S100A8 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Affinity Purification
• Concentration: 0.36 mg/mL (varies by lot)

• Applicable Applications for anti-S100A8 antibody: Immunohistochemistry (IHC)
• Application Notes: IHC 1:100-1:200
• Immunogen: Recombinant Human Protein S100-A8 protein
• Conjugation: Unconjugated
• Buffer: PBS with 0.05% Proclin300 and 50% glycerol, pH7.4.
• Preparation and Storage: Store at -20 degree C. Avoid freeze / thaw cycles.

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded Human cervix using S100A8 Polyclonal Antibody at dilution of 1:100(100×))

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded Human cervix using S100A8 Polyclonal Antibody at dilution of 1:100(400×))

Related Product Information for anti-S100A8 antibody

The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in the inhibition of casein kinase and as a cytokine. Altered expression of this protein is associated with the disease cystic fibrosis. Multiple transcript variants encoding different isoforms have been found for this gene.

Product Categories/Family for anti-S100A8 antibody

Cancer; Neuroscience

NCBI and Uniprot Product Information

• UniProt Accession #: P05109
• Protein Family: Protein

NCBI Description

NF-kappa-B is a ubiquitous transcription factor involved in several biological processes. It is held in the cytoplasm in an inactive state by specific inhibitors. Upon degradation of the inhibitor, NF-kappa-B moves to the nucleus and activates transcription of specific genes. NF-kappa-B is composed of NFKB1 or NFKB2 bound to either REL, RELA, or RELB. The most abundant form of NF-kappa-B is NFKB1 complexed with the product of this gene, RELA. Four transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011]

Uniprot Description

NF-kappa-B is a pleiotropic transcription factor present in almost all cell types and is the endpoint of a series of signal transduction events that are initiated by a vast array of stimuli related to many biological processes such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52. The heterodimeric RELA-NFKB1 complex appears to be most abundant one. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively. The NF-kappa-B heterodimeric RELA-NFKB1 and RELA-REL complexes, for instance, function as transcriptional activators. NF-kappa-B is controlled by various mechanisms of post-translational modification and subcellular compartmentalization as well as by interactions with other cofactors or corepressors. NF-kappa-B complexes are held in the cytoplasm in an inactive state complexed with members of the NF-kappa-B inhibitor (I-kappa-B) family. In a conventional activation pathway, I-kappa-B is phosphorylated by I-kappa-B kinases (IKKs) in response to different activators, subsequently degraded thus liberating the active NF-kappa-B complex which translocates to the nucleus. The inhibitory effect of I-kappa-B on NF-kappa-B through retention in the cytoplasm is exerted primarily through the interaction with RELA. RELA shows a weak DNA-binding site which could contribute directly to DNA binding in the NF-kappa-B complex. Beside its activity as a direct transcriptional activator, it is also able to modulate promoters accessibility to transcription factors and thereby indirectly regulate gene expression. Associates with chromatin at the NF-kappa-B promoter region via association with DDX1. Essential for cytokine gene expression in T-cells (PubMed:15790681). The NF-kappa-B homodimeric RELA-RELA complex appears to be involved in invasin-mediated activation of IL-8 expression.