Western Blot (WB)
(Western blot analysis of anti-hRYK-W576 Pab pre-incubated without(lane 1) and with(lane 2) blocking peptide in 293 cell line lysate. hRYK-W576(arrow) was detected using the purified Pab.)
Rabbit RYK Polyclonal Antibody | anti-RYK antibody
RYK Antibody (C-term)
Western Blot (WB)
(Western blot analysis of anti-hRYK-W576 Pab pre-incubated without(lane 1) and with(lane 2) blocking peptide in 293 cell line lysate. hRYK-W576(arrow) was detected using the purified Pab.)
Western Blot (WB)
(Western blot analysis of anti-hRYK-W576 Pab pre-incubated without(lane 1) and with(lane 2) blocking peptide in 293 cell line lysate. hRYK-W576(arrow) was detected using the purified Pab.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)
Katso, R.M., et al., Mol. Cell. Biol. 19(9):6427-6440 (1999).
Wang, X.C., et al., Mol. Med. 2(2):189-203 (1996).
Tamagnone, L., et al., Oncogene 8(7):2009-2014 (1993).
Stacker, S.A., et al., Oncogene 8(5):1347-1356 (1993).
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is an atypical member of the family of growth factor receptor protein tyrosine kinases, differing from other members at a number of conserved residues in the activation and nucleotide binding domains. This gene product belongs to a subfamily whose members do not appear to be regulated by phosphorylation in the activation segment. It has been suggested that mediation of biological activity by recruitment of a signaling-competent auxiliary protein may occur through an as yet uncharacterized mechanism. The encoded protein has a leucine-rich extracellular domain with a WIF-type Wnt binding region, a single transmembrane domain, and an intracellular tyrosine kinase domain. This protein is involved in stimulating Wnt signaling pathways such as the regulation of axon pathfinding. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Feb 2012]
Uniprot Description
RYK: a receptor tyrosine kinase of the Ryk family. Associates with, and may modulate the signaling of, EphB2 and EphB3. Expressed in nestin-positive progenitor cells and neurons, some oligodendrocytes, O-2A progenitor cells, and type-2 astrocytes in developing CNS.
Protein type: Protein kinase, TK; Kinase, protein; Membrane protein, integral; Protein kinase, tyrosine (receptor); EC 2.7.10.1; TK group; Ryk family
Chromosomal Location of Human Ortholog: 3q22
Cellular Component: membrane; integral to plasma membrane; cytoplasm; plasma membrane; integral to membrane; nucleus
Molecular Function: protein binding; transmembrane receptor activity; protein-tyrosine kinase activity; transmembrane receptor protein tyrosine kinase activity; ATP binding
Biological Process: corpus callosum development; neuron differentiation; axon guidance; Wnt receptor signaling pathway; peptidyl-tyrosine phosphorylation; positive regulation of MAPKKK cascade; signal transduction; protein amino acid phosphorylation; neurite development
Research Articles on RYK
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Product Notes
The RYK ryk (Catalog #AAA9205094) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 561-591. The RYK Antibody (C-term) reacts with Human (Predicted Reactivity: Mouse) and may cross-react with other species as described in the data sheet. AAA Biotech's RYK can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC). WB~~1:1000. Researchers should empirically determine the suitability of the RYK ryk for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RYK, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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