Rabbit anti-Human RYK Polyclonal Antibody | anti-RYK antibody

RYK, CT (RYK, JTK5A, Tyrosine-protein kinase RYK) (FITC)

Cat. Num. AAA6344593

• Gene Names: RYK; JTK5; RYK1; JTK5A; D3S3195
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein G Affinity Chromatography.
• Synonyms: RYK; Polyclonal Antibody; CT (RYK; JTK5A; Tyrosine-protein kinase RYK) (FITC); Tyrosine-protein kinase RYK; anti-RYK antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-RYK antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:50-100; FLISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: RYK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 569~599 amino acids from the C-terminal region of human RYK.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of anti-hRYK-W576 Pab pre-incubated without(lane 1) and with(lane 2) blocking peptide in 293 cell line lysate. hRYK-W576(arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

Related Product Information for anti-RYK antibody

RYK is an atypical member of the family of growth factor receptor protein tyrosine kinases, differing from other members at a number of conserved residues in the activation and nucleotide binding domains. This gene product belongs to a subfamily whose members do not appear to be regulated by phosphorylation in the activation segment. It has been suggested that mediation of biological activity by recruitment of a signaling-competent auxiliary protein may occur through an as yet uncharacterized mechanism. A nine nucleotide insertion in some transcripts results in the SLG variant. It is not established whether this is a product of alternative splicing or a second gene, since evidence for a second gene or pseudogene on chromosome 17 exists.

Product Categories/Family for anti-RYK antibody

Antibodies; Protein Kinases

References

Trivier, E., et al., J. Biol. Chem. 277(25):23037-23043 (2002). Katso, R.M., et al., Mol. Cell. Biol. 19(9):6427-6440 (1999). Wang, X.C., et al., Mol. Med. 2(2):189-203 (1996). Tamagnone, L., et al., Oncogene 8(7):2009-2014 (1993). Stacker, S.A., et al., Oncogene 8(5):1347-1356 (1993).

NCBI and Uniprot Product Information

• NCBI GI #: 116805325
• NCBI GeneID: 6259
• NCBI Accession #: NP_001005861.1
• NCBI GenBank Nucleotide #: NM_001005861
• UniProt Accession #: P34925; Q04696
• Molecular Weight: ~66 kDa
• NCBI Official Full Name: Homo sapiens receptor-like tyrosine kinase (RYK), transcript variant 1, mRNA
• NCBI Official Synonym Full Names: receptor-like tyrosine kinase
• NCBI Official Symbol: RYK
• NCBI Official Synonym Symbols: JTK5; RYK1; JTK5A; D3S3195
• NCBI Protein Information: tyrosine-protein kinase RYK; hydroxyaryl-protein kinase; JTK5A protein tyrosine kinase
• UniProt Protein Name: Tyrosine-protein kinase RYK
• Protein Family: Tyrosine-protein kinase
• UniProt Gene Name: RYK
• UniProt Synonym Gene Names: JTK5A
• UniProt Entry Name: RYK_HUMAN

NCBI Description

The protein encoded by this gene is an atypical member of the family of growth factor receptor protein tyrosine kinases, differing from other members at a number of conserved residues in the activation and nucleotide binding domains. This gene product belongs to a subfamily whose members do not appear to be regulated by phosphorylation in the activation segment. It has been suggested that mediation of biological activity by recruitment of a signaling-competent auxiliary protein may occur through an as yet uncharacterized mechanism. The encoded protein has a leucine-rich extracellular domain with a WIF-type Wnt binding region, a single transmembrane domain, and an intracellular tyrosine kinase domain. This protein is involved in stimulating Wnt signaling pathways such as the regulation of axon pathfinding. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Feb 2012]

Uniprot Description

Function: May be a coreceptor along with FZD8 of Wnt proteins, such as WNT1, WNT3, WNT3A and WNT5A. Involved in neuron differentiation, axon guidance, corpus callosum establishment and neurite outgrowth. In response to WNT3 stimulation, receptor C-terminal cleavage occurs in its transmembrane region and allows the C-terminal intracellular product to translocate from the cytoplasm to the nucleus where it plays a crucial role in neuronal development. Ref.5

Catalytic activity: ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.

Subunit structure: Interacts with DVL1 (via PDZ domain). Ref.5

Subcellular location: Membrane; Single-pass type I membrane protein

By similarity. Nucleus

By similarity. Cytoplasm

By similarity. Note: In cells that have undergone neuronal differentiation, the C-terminal cleaved part is translocated from the cytoplasm to the nucleus

By similarity.

Tissue specificity: Observed in all the tissues examined.

Domain: The extracellular WIF domain is responsible for Wnt binding

By similarity.

Post-translational modification: Proteolytically cleaved, in part by presenilin, in response to WNT3 stimulation. Cleavage occurs during neuronal differentiation. Ref.6

Sequence similarities: Belongs to the protein kinase superfamily. Tyr protein kinase family.Contains 1 protein kinase domain.Contains 1 WIF domain.

Caution: According to some authors, has impaired kinase activity (Ref.4).