Rabbit RYK Polyclonal Antibody | anti-RYK antibody

RYK Antibody

Cat. Num. AAA9212723

• Gene Names: RYK; JTK5; RYK1; JTK5A; D3S3195
• Reactivity: Human, mouse, rat
• Applications: Western Blot, ELISA, Immunohistochemistry
• Purity: Purified Rabbit Polyclonal Antibody (Pab)
• Synonyms: RYK; Polyclonal Antibody; RYK Antibody; Tyrosine-protein kinase RYK; JTK5A; anti-RYK antibody

• Host: Rabbit
• Reactivity: Human, mouse, rat
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Specificity: This RYK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 160-190 amino acids from human RYK.
• Purity/Purification: Purified Rabbit Polyclonal Antibody (Pab)
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Sequence Positions: 160-190
• Sequence Length: 610

• Applicable Applications for anti-RYK antibody: Western Blot (WB), ELISA (EIA), Immunohistochemistry-Paraffin (IHC-P)
• Application Notes: WB~~1:1000; IHC-P~~1:50~100
• Function: May be a coreceptor along with FZD8 of Wnt proteins, such as WNT1, WNT3, WNT3A and WNT5A. Involved in neuron differentiation, axon guidance, corpus callosum establishment and neurite outgrowth. In response to WNT3 stimulation, receptor Cterminal cleavage occurs in its transmembrane region and allows the C-terminal intracellular product to translocate from the cytoplasm to the nucleus where it plays a crucial role in neuronal development.
• Cellular Location: Membrane; Single-pass type I membrane protein. Nucleus. Cytoplasm. Note=In cells that have undergone neuronal differentiation, the C-terminal cleaved part is translocated from the cytoplasm to the nucleus.
• Tissue Location: Observed in all the tissues examined.
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with RYK Antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Western Blot (WB)

(Western blot analysis of lysate from mouse NIH/3T3 cell line, using RYK Antibody (N175). MBS9212723 was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20ug.)

Western Blot (WB)

(Western blot analysis of lysate from human ovary tissue lysate, using RYK Antibody (N175). MBS9212723 was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20ug.)

Western Blot (WB)

(Western blot analysis of lysate from human ovary tissue lysate, using RYK Antibody (N175). MBS9212723 was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20ug.)

Western Blot (WB)

(Western blot analysis of lysates from mouse lung and ovary tissue lysate(from left to right), using RYK Antibody (N175). MBS9212723 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.)

Western Blot (WB)

(The anti-RYK Pab is used in Western blot to detect RYK in Jurkat cell lysate.)

Western Blot (WB)

(Western blot analysis of RYK (arrow) using rabbit polyclonal RYK Antibody. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the RYK gene (Lane 2) (Origene Technologies).)

Related Product Information for anti-RYK antibody

RYK is an atypical member of the family of growth factor receptor protein tyrosine kinases, differing from other members at a number of conserved residues in the activation and nucleotide binding domains. This gene product belongs to a subfamily whose members do not appear to be regulated by phosphorylation in the activation segment. It has been suggested that mediation of biological activity by recruitment of a signaling-competent auxiliary protein may occur through an as yet uncharacterized mechanism. A nine nucleotide insertion in some transcripts results in the SLG variant. It is not established whether this is a product of alternative splicing or a second gene, since evidence for a second gene or pseudogene on chromosome 17 exists.

Product Categories/Family for anti-RYK antibody

Signal Transduction

References

Trivier, E., et al., J. Biol. Chem. 277(25):23037-23043 (2002). Katso, R.M., et al., Mol. Cell. Biol. 19(9):6427-6440 (1999). Wang, X.C., et al., Mol. Med. 2(2):189-203 (1996). Tamagnone, L., et al., Oncogene 8(7):2009-2014 (1993). Stacker, S.A., et al., Oncogene 8(5):1347-1356 (1993).

NCBI and Uniprot Product Information

• NCBI GI #: 54607020
• NCBI GeneID: 6259
• NCBI Accession #: NP_001005861.1; NP_002949.2
• NCBI GenBank Nucleotide #: NM_001005861.2
• UniProt Accession #: P34925; Q04696
• NCBI Official Full Name: tyrosine-protein kinase RYK isoform 1
• NCBI Official Synonym Full Names: receptor-like tyrosine kinase
• NCBI Official Symbol: RYK
• NCBI Official Synonym Symbols: JTK5; RYK1; JTK5A; D3S3195
• NCBI Protein Information: tyrosine-protein kinase RYK
• UniProt Protein Name: Tyrosine-protein kinase RYK
• Protein Family: Tyrosine-protein kinase
• UniProt Gene Name: RYK
• UniProt Synonym Gene Names: JTK5A
• UniProt Entry Name: RYK_HUMAN

NCBI Description

The protein encoded by this gene is an atypical member of the family of growth factor receptor protein tyrosine kinases, differing from other members at a number of conserved residues in the activation and nucleotide binding domains. This gene product belongs to a subfamily whose members do not appear to be regulated by phosphorylation in the activation segment. It has been suggested that mediation of biological activity by recruitment of a signaling-competent auxiliary protein may occur through an as yet uncharacterized mechanism. The encoded protein has a leucine-rich extracellular domain with a WIF-type Wnt binding region, a single transmembrane domain, and an intracellular tyrosine kinase domain. This protein is involved in stimulating Wnt signaling pathways such as the regulation of axon pathfinding. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Feb 2012]

Uniprot Description

RYK: a receptor tyrosine kinase of the Ryk family. Associates with, and may modulate the signaling of, EphB2 and EphB3. Expressed in nestin-positive progenitor cells and neurons, some oligodendrocytes, O-2A progenitor cells, and type-2 astrocytes in developing CNS.

Protein type: Protein kinase, TK; Kinase, protein; EC 2.7.10.1; Protein kinase, tyrosine (receptor); Membrane protein, integral; TK group; Ryk family

Chromosomal Location of Human Ortholog: 3q22

Cellular Component: membrane; integral to plasma membrane; cytoplasm; plasma membrane; integral to membrane; nucleus

Molecular Function: protein binding; transmembrane receptor activity; protein-tyrosine kinase activity; transmembrane receptor protein tyrosine kinase activity; ATP binding

Biological Process: corpus callosum development; neuron differentiation; axon guidance; Wnt receptor signaling pathway; peptidyl-tyrosine phosphorylation; positive regulation of MAPKKK cascade; signal transduction; protein amino acid phosphorylation; neurite development