Mouse PIIICP (c-terminal Procollagen III propeptide) CLIA Kit | PIIICP clia kit

Mouse PIIICP (c-terminal Procollagen III propeptide) CLIA Kit

Cat. Num. AAA2532692

• Gene Names: COL3A1; EDS4A
• Reactivity: Mouse
• Synonyms: PIIICP (c-terminal Procollagen III propeptide); Mouse PIIICP (c-terminal Procollagen III propeptide) CLIA Kit; PIIICP clia kit

• Reactivity: Mouse
• Specificity: This kit recognizes natural and recombinant PIIICP. No significant cross-reactivity or interference between PIIICP and analogues was observed.
• Sequence Length: 245

• Assay Type: Sandwich
• Detection Range: 7.813-500pg/mL
• Sensitivity: Min: 4.688pg/mL; Max: 500pg/mL

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for PIIICP clia kit

Intended Uses: This CLIA kit applies to the in vitro quantitative determination of PIIICP concentrations in serum, plasma and other biological fluids.

Principle of the Assay: This kit uses Sandwich-CLIA as the method. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to PIIICP. Standards or samples are added to the appropriate micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for PIIICP and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain PIIICP, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured spectrophotometrically by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of PIIICP. You can calculate the concentration of PIIICP in the samples by comparing the RLU value of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 1335249
• NCBI GeneID: 1281
• UniProt Accession #: P02461; P78429; Q15112; Q16403; Q53S91; Q541P8; Q6LDB3; Q6LDJ2; Q6LDJ3; Q7KZ56; D2JYH5; D3DPH4
• Molecular Weight: 111,907 Da
• NCBI Official Full Name: carboxy-propeptide of alpha 1 (III) procollagen, partial
• NCBI Official Synonym Full Names: collagen, type III, alpha 1
• NCBI Official Symbol: COL3A1
• NCBI Official Synonym Symbols: EDS4A
• NCBI Protein Information: collagen alpha-1(III) chain
• UniProt Protein Name: Collagen alpha-1(III) chain
• UniProt Gene Name: COL3A1
• UniProt Entry Name: CO3A1_HUMAN

NCBI Description

This gene encodes the pro-alpha1 chains of type III collagen, a fibrillar collagen that is found in extensible connective tissues such as skin, lung, uterus, intestine and the vascular system, frequently in association with type I collagen. Mutations in this gene are associated with Ehlers-Danlos syndrome types IV, and with aortic and arterial aneurysms. Two transcripts, resulting from the use of alternate polyadenylation signals, have been identified for this gene. [provided by R. Dalgleish, Feb 2008]

Uniprot Description

CO3A1: Collagen type III occurs in most soft connective tissues along with type I collagen. Defects in COL3A1 are a cause of Ehlers-Danlos syndrome type 3 (EDS3); also known as benign hypermobility syndrome. EDS is a connective tissue disorder characterized by hyperextensible skin, atrophic cutaneous scars due to tissue fragility and joint hyperlaxity. EDS3 is a form of Ehlers-Danlos syndrome characterized by marked joint hyperextensibility without skeletal deformity. Defects in COL3A1 are the cause of Ehlers-Danlos syndrome type 4 (EDS4). EDS is a connective tissue disorder characterized by hyperextensible skin, atrophic cutaneous scars due to tissue fragility and joint hyperlaxity. EDS4 is the most severe form of the disease. It is characterized by the joint and dermal manifestations as in other forms of the syndrome, characteristic facial features (acrogeria) in most patients, and by proneness to spontaneous rupture of bowel and large arteries. The vascular complications may affect all anatomical areas. Defects in COL3A1 are a cause of susceptibility to aortic aneurysm abdominal (AAA). AAA is a common multifactorial disorder characterized by permanent dilation of the abdominal aorta, usually due to degenerative changes in the aortic wall. Histologically, AAA is characterized by signs of chronic inflammation, destructive remodeling of the extracellular matrix, and depletion of vascular smooth muscle cells. Belongs to the fibrillar collagen family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Extracellular matrix; Secreted; Motility/polarity/chemotaxis; Cell adhesion

Chromosomal Location of Human Ortholog: 2q31

Cellular Component: extracellular matrix; extracellular space; endoplasmic reticulum lumen; extracellular region; collagen type III

Molecular Function: integrin binding; protein binding; metal ion binding; extracellular matrix structural constituent; platelet-derived growth factor binding; SMAD binding

Biological Process: skin development; integrin-mediated signaling pathway; axon guidance; receptor-mediated endocytosis; platelet activation; extracellular matrix organization and biogenesis; collagen fibril organization; wound healing; cell-matrix adhesion; heart development; negative regulation of immune response; positive regulation of Rho protein signal transduction; extracellular matrix disassembly; collagen catabolic process; response to radiation; gut development; response to mechanical stimulus; transforming growth factor beta receptor signaling pathway; response to cytokine stimulus; fibril organization and biogenesis; peptide cross-linking; cerebral cortex development; skeletal development; aging

Disease: Ehlers-danlos Syndrome, Type Iv, Autosomal Dominant; Ehlers-danlos Syndrome, Type Iii