Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Rabbit anti-Gallus Nuclear Factor Kappa B2 Polyclonal Antibody | anti-NFkB2 antibody
Polyclonal Antibody to Nuclear Factor Kappa B2 (NFkB2)
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- PYLV IIEQPKQRGF RFRYVCEGPS HGGLPGASSE KGHKTYPTVK ICNYEGMARI EVDLVTHSDP PRVHAHSLVG KQCNEAGNCV AIVGPKDMTA QFSNLGVLHV TKKNMMEIMK EKLKKQKTRN TNGLLTEAEL REIELEAKEL KKVMDLSIVR LRFTAYLRDS SGNFTLALQP VISDPIHDSK SPGASNLKIS RMDKTAGSVR GGDEVYLLCD KVQKDDIEVR FYEDDENGWQ ASGDFSPTDV HKQYAIVFRT PPYHKPKIDR PVTVFLQLKR KRGGDVSDSK QFTYYPVVED KEEVERNRKK VL
Immunohistochemistry: 5-20ug/mL;1:15-55
Immunocytochemistry: 5-20ug/mL;1:15-55
Optimal working dilutions must be determined by end user.
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
NCBI and Uniprot Product Information
Uniprot Description
NF-kappa-B is a pleiotropic transcription factor present in almost all cell types and is the endpoint of a series of signal transduction events that are initiated by a vast array of stimuli related to many biological processes such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively. NF-kappa-B is controlled by various mechanisms of post-translational modification and subcellular compartmentalization as well as by interactions with other cofactors or corepressors. NF-kappa-B complexes are held in the cytoplasm in an inactive state complexed with members of the NF-kappa-B inhibitor (I-kappa-B) family. In a conventional activation pathway, I-kappa-B is phosphorylated by I-kappa-B kinases (IKKs) in response to different activators, subsequently degraded thus liberating the active NF-kappa-B complex which translocates to the nucleus. In a non-canonical activation pathway, the MAP3K14-activated CHUK/IKKA homodimer phosphorylates NFKB2/p100 associated with RelB, inducing its proteolytic processing to NFKB2/p52 and the formation of NF-kappa-B RelB-p52 complexes. The NF-kappa-B heterodimeric RelB-p52 complex is a transcriptional activator. NFKB2 appears to have dual functions such as cytoplasmic retention of attached NF-kappa-B proteins by p100 and generation of p52 by a cotranslational processing. The proteasome-mediated process ensures the production of both p52 and p100 and preserves their independent function. p52 binds to the kappa-B consensus sequence 5'-GGRNNYYCC-3', located in the enhancer region of genes involved in immune response and acute phase reactions. In concert with RELB, may play a role in the regulation of the circadian clock ().
Research Articles on NFkB2
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Product Notes
The NFkB2 nfkb2 (Catalog #AAA2026550) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Nuclear Factor Kappa B2 (NFkB2) reacts with Gallus and may cross-react with other species as described in the data sheet. AAA Biotech's Nuclear Factor Kappa B2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA). Western blotting: 0.5-2ug/mL;1:150-550 Immunohistochemistry: 5-20ug/mL;1:15-55 Immunocytochemistry: 5-20ug/mL;1:15-55 Optimal working dilutions must be determined by end user. Researchers should empirically determine the suitability of the NFkB2 nfkb2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with two N-terminal Tags, His-tag and T7-tag and its sequence is listed below. MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- PYLV IIEQPKQRGF RFRYVCEGPS HGGLPGASSE KGHKTYPTVK ICNYEGMARI EVDLVTHSDP PRVHAHSLVG KQCNEAGNCV AIVGPKDMTA QFSNLGVLHV TKKNMMEIMK EKLKKQKTRN TNGLLTEAEL REIELEAKEL KKVMDLSIVR LRFTAYLRDS SGNFTLALQP VISDPIHDSK SPGASNLKIS RMDKTAGSVR GGDEVYLLCD KVQKDDIEVR FYEDDENGWQ ASGDFSPTDV HKQYAIVFRT PPYHKPKIDR PVTVFLQLKR KRGGDVSDSK QFTYYPVVED KEEVERNRKK VL. It is sometimes possible for the material contained within the vial of "Nuclear Factor Kappa B2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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