Western Blot (WB)
(Western BlotPositive WB detected in: Hela whole cell lysate, 293T whole cell lysate, K562 whole cell lysate, SH-SY5Y whole cell lysateAll lanes: RUFY3 antibody at 3.71ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 53, 56, 71, 65 KDaObserved band size: 53 KDa)
Rabbit anti-Human RUFY3 Polyclonal Antibody | anti-RUFY3 antibody
RUFY3 Antibody
IF: 1:50-1:200
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Western Blot (WB)
(Western BlotPositive WB detected in: Hela whole cell lysate, 293T whole cell lysate, K562 whole cell lysate, SH-SY5Y whole cell lysateAll lanes: RUFY3 antibody at 3.71ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 53, 56, 71, 65 KDaObserved band size: 53 KDa)
Western Blot (WB)
(Western BlotPositive WB detected in: Hela whole cell lysate, 293T whole cell lysate, K562 whole cell lysate, SH-SY5Y whole cell lysateAll lanes: RUFY3 antibody at 3.71ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 53, 56, 71, 65 KDaObserved band size: 53 KDa)
Immunofluorescence (IF)
(Immunofluorescence staining of Hela cells with MBS7109280 at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4 degree C.The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).)
Immunofluorescence (IF)
(Immunofluorescence staining of Hela cells with MBS7109280 at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4 degree C.The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a RPIP8, UNC-14, and NESCA domain-containing protein that is required for maintenance of neuronal polarity. In addition, it has been implicated in mediation of gastric cancer cell migration and invasion via interaction with P21-activated kinase-1, which promotes its expression. The encoded protein localizes to F-actin-enriched invadopodia to induce formation of protrusions, thereby facilitating cell migration. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2016]
Uniprot Description
Plays a role in the generation of neuronal polarity formation and axon growth (). Implicated in the formation of a single axon by developing neurons (). May inhibit the formation of additional axons by inhibition of PI3K in minor neuronal processes (). Plays a role in the formation of F-actin-enriched protrusive structures at the cell periphery (PubMed:25766321). Plays a role in cytoskeletal organization by regulating the subcellular localization of FSCN1 and DBN1 at axonal growth cones (). Promotes gastric cancer cell migration and invasion in a PAK1-dependent manner (PubMed:25766321).
Research Articles on RUFY3
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Product Notes
The RUFY3 rufy3 (Catalog #AAA7109280) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The RUFY3 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's RUFY3 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunofluorescence (IF). WB: 1:500-1:5000 IF: 1:50-1:200. Researchers should empirically determine the suitability of the RUFY3 rufy3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RUFY3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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