SDS-PAGE
Creatinine amidohydrolase Recombinant Protein | crnA recombinant protein
Recombinant Pseudomonas putida (Arthrobacter siderocapsulatus) Creatinine amidohydrolase
Purity
Greater or equal to 85% purity as determined by SDS-PAGE.
Synonyms
Creatinine amidohydrolase; Recombinant Pseudomonas putida (Arthrobacter siderocapsulatus) Creatinine amidohydrolase; Creatininase; crnA recombinant protein
Host
E Coli or Yeast or Baculovirus or Mammalian Cell
Purity/Purification
Greater or equal to 85% purity as determined by SDS-PAGE.
Form/Format
Lyophilized or liquid (Format to be determined during the manufacturing process)
Sequence Positions
1-260aa; Full Length
Sequence
MSKSVFVGELTWKEYEARVAAGDCVLMLPVGALEQHGHHMCMNVDVLLPTAVCKRVAERIGALVMPGLQYGYKSQQKSGGGNHFPGTTSLDGATLTGTVQDIIRELARHGARRLVLMNGHYENSMFIVEGIDLALRELRYAGIQDFKVVVLSYWDFVKDPAVIQQLYPEGFLGWDIEHGGVFETSLMLALYPDLVDLDRVVDHPPATFPPYDVFPVDPARTPAPGTLSSAKTASREKGELILEVCVQGIADAIREEFPPT
Sequence Length
260
Preparation and Storage
Store at -20 degree C, for extended storage, conserve at -20 degree C or -80 degree C.
SDS-PAGE
SDS-PAGE
Related Product Information for crnA recombinant protein
Cyclic amidohydrolase that catalyzes the reversible conversion of creatinine to creatine. Is also active toward glycocyamidine, though the reaction rate is very low, but it is completely inert toward hydantoin and its derivatives.
References
Cloning of the creatinine amidohydrolase gene from Pseudomonas sp. PS-7.Yamamoto K., Oka M., Kikuchi T., Emi S.Biosci. Biotechnol. Biochem. 59:1331-1332(1995)
Creatinine amidohydrolase (creatininase)
from Pseudomonas putida. Purification and some properties.Rikitake K., Oka I., Ando M., Yoshimoto T., Tsuru D.J. Biochem. 86:1109-1117(1979)
Crystal structure of creatininase from Pseudomonas putida
a novel fold and a case of convergent evolution.Beuth B., Niefind K., Schomburg D.J. Mol. Biol. 332:287-301(2003)
Crystal structures of creatininase reveal the substrate binding site and provide an insight into the catalytic mechanism.Yoshimoto T., Tanaka N., Kanada N., Inoue T., Nakajima Y., Haratake M., Nakamura K.T., Xu Y., Ito K.J. Mol. Biol. 337:399-416(2004)
Substitution of Glu122 by glutamine revealed the function of the second water molecule as a proton donor in the binuclear metal enzyme creatininase.Yamashita K., Nakajima Y., Matsushita H., Nishiya Y., Yamazawa R., Wu Y.F., Matsubara F., Oyama H., Ito K., Yoshimoto T.J. Mol. Biol. 396:1081-1096(2010)
NCBI and Uniprot Product Information
NCBI GI #
Molecular Weight
55.6 kDa
NCBI Official Full Name
Creatinine amidohydrolase
UniProt Protein Name
Creatinine amidohydrolase
Protein Family
UniProt Gene Name
crnA
UniProt Entry Name
CRNA_PSEPU
Uniprot Description
Cyclic amidohydrolase that catalyzes the reversible conversion of creatinine to creatine. Is also active toward glycocyamidine, though the reaction rate is very low, but it is completely inert toward hydantoin and its derivatives.
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Product Notes
The crnA crna (Catalog #AAA1293060) is a Recombinant Protein produced from E Coli or Yeast or Baculovirus or Mammalian Cell and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 1-260aa; Full Length. The amino acid sequence is listed below: MSKSVFVGEL TWKEYEARVA AGDCVLMLPV GALEQHGHHM CMNVDVLLPT AVCKRVAERI GALVMPGLQY GYKSQQKSGG GNHFPGTTSL DGATLTGTVQ DIIRELARHG ARRLVLMNGH YENSMFIVEG IDLALRELRY AGIQDFKVVV LSYWDFVKDP AVIQQLYPEG FLGWDIEHGG VFETSLMLAL YPDLVDLDRV VDHPPATFPP YDVFPVDPAR TPAPGTLSSA KTASREKGEL ILEVCVQGIA DAIREEFPPT. It is sometimes possible for the material contained within the vial of "Creatinine amidohydrolase, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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