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Typical Testing Data/Standard Curve (for reference only)

Human TRNA Methyltransferase 61B (TRMT61B) RTU ELISA Kit | TRMT61B rtu elisa kit

Human TRNA Methyltransferase 61B (TRMT61B) ELISA Kit

Reactivity
Human
Synonyms
TRNA Methyltransferase 61B (TRMT61B); Human TRNA Methyltransferase 61B (TRMT61B) ELISA Kit; TRMT61B rtu elisa kit
Ordering
For Research Use Only!
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of TRF. No significant cross-reactivity or interference between TRF and analogues was observed.
Sequence Length
477
Samples
Bovine Serum, Plasma Or Other Biological Fluids
Assay Type
Quantitative Sandwich
Detection Range
1.56-100ng/mL
Sensitivity
0.70ng/mL.
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level TRF were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level TRF were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
Preparation and Storage
Stable for 12 months.

Typical Testing Data/Standard Curve (for reference only)

Typical Testing Data/Standard Curve (for reference only)
Related Product Information for TRMT61B rtu elisa kit
Intended Uses: The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of TRF in bovine serum, plasma or other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to TRF. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to TRF. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain TRF, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of TRF in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
52,965 Da
NCBI Official Full Name
tRNA (adenine(58)-N(1))-methyltransferase, mitochondrial
NCBI Official Synonym Full Names
tRNA methyltransferase 61B
NCBI Official Symbol
TRMT61B
NCBI Protein Information
tRNA (adenine(58)-N(1))-methyltransferase, mitochondrial
UniProt Protein Name
tRNA (adenine(58)-N(1))-methyltransferase, mitochondrial
UniProt Gene Name
TRMT61B

Uniprot Description

Methyltransferase that catalyzes the formation of N1-methyladenine at position 58 (m1A58) in various tRNAs in mitochondrion, including tRNA(Leu) (deciphering codons UUA or UUG), tRNA(Lys) and tRNA(Ser) (deciphering codons UCA, UCU, UCG or UCC) (PubMed:23097428). Catalyzes the formation of 1-methyladenosine at position 947 of mitochondrial 16S ribosomal RNA and this modification is most likely important for mitoribosomal structure and function (PubMed:27631568). In addition to tRNA N1-methyltransferase activity, also acts as a mRNA N1-methyltransferase by mediating methylation of adenosine residues at the N1 position of MT-ND5 mRNA, leading to interfere with mitochondrial translation (PubMed:29107537).

Research Articles on TRMT61B

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Product Notes

The Human TRMT61B trmt61b (Catalog #AAA4503822) is a RTU ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA4503822 RTU ELISA Kit recognizes Human TRMT61B. It is sometimes possible for the material contained within the vial of "TRNA Methyltransferase 61B (TRMT61B), RTU ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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