Rabbit anti-Human HBB Polyclonal Antibody

HBB, CT (HBB, Hemoglobin subunit beta, Beta-globin, Hemoglobin beta chain, LVV-hemorphin-7)

Cat. Num. AAA648109

• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry, Flow Cytometry, Functional Assay
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: HBB; Polyclonal Antibody; CT (HBB; Hemoglobin subunit beta; Beta-globin; Hemoglobin beta chain; LVV-hemorphin-7); Anti -HBB; anti-HBB antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human HBB at ~16kD.
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.

• Applicable Applications for anti-HBB antibody: Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
• Application Notes: Suitable for use in Western Blot, Immunohistochemistry and Flow Cytometry. Othre applications not tested.; Dilution: Western Blot: 1:100-500; Flow Cytometry: 1:10-50; Immunohistochemistry (FFPE): 10-50; Optimal dilutions to be determined by the researcher
• Immunogen: Synthetic peptide corresponding to a region near aa87-115 from the C-terminal of human HBB, conjugated to KLH (P68871). Cellular Localization: Red blood cells. Species Sequence Homology: bovine, chicken, mouse, porcine, rat and rabbit
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in K562 cell line lysates (35ug/lane) using MBS648109. This demonstrates that MBS648109 detected the HBB protein (arrow).)

Testing Data

(All lanes: MBS648109 (1:2000) Lane 1: human heart lysate Lane 2: human liver lysate Lane 3: K562 whole cell lysate Lysates/proteins at 20ug/lane. Secondary Goat Anti-Rabbit IgG, (H+L), peroxidase conjugated at 1:10,000. Predicted band size: 16kD. Blocking/Dilution buffer: 5% NFDM/TBST.)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue using MBS648109 followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of MBS648109 for immunohistochemistry.)

Flow Cytometry (FC/FACS)

(Flow Cytometric analysis of K562 cells using MBS648109 (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-HBB antibody

The alpha (HBA) and beta (HBB) loci determine the structure of the 2 types of polypeptide chains in adult hemoglobin, Hb A. The normal adult hemoglobin tetramer consists of two alpha chains and two beta chains. Mutant beta globin causes sickle cell anemia. Absence of beta chain causes beta-zero-thalassemia. Reduced amounts of detectable beta globin causes beta-plus-thalassemia. The order of the genes in the beta-globin cluster is 5'-epsilon -- gamma-G -- gamma-A -- delta -- beta--3'.

Product Categories/Family for anti-HBB antibody

Antibodies; Abs to Hemoglobin

NCBI and Uniprot Product Information

• NCBI GI #: 89145742
• NCBI Official Full Name: Hbb
• Protein Family: Hemoglobin

Uniprot Description

Histone-like DNA-binding protein which is capable of wrapping DNA to stabilize it, and thus to prevent its denaturation under extreme environmental conditions.