Rabbit RRAGB Polyclonal Antibody | anti-RRAGB antibody

RRAGB Antibody

Cat. Num. AAA9605847

• Gene Names: RRAGB; RAGB; bA465E19.1
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
• Synonyms: RRAGB; Polyclonal Antibody; RRAGB Antibody; Adenovirus E3 14.7 kDa-interacting protein 1; FIP1; Rag A; RAg B; RagA; RAGB; Ras related GTP binding protein A; Ras-related GTP binding protein B; anti-RRAGB antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: RRAGB antibody detects endogenous levels of total RRAGB
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
• Form/Format: Liquid; Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 346

• Applicable Applications for anti-RRAGB antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:500-1:1000
• Immunogen: A synthesized peptide
• Subcellular Location: Cytoplasmic and Nuclear: RRAGA may shuttle between the cytoplasm and nucleus, depending on the bound nucleotide state and colocalizes in vivo with adenovirus E3-14.7K. RRAGB is cytoplasmic.
• Conjugation: Unconjugated
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Western blot analysis of RRAGB expression in various lysates)

Related Product Information for anti-RRAGB antibody

Function: Guanine nucleotide-binding protein that plays a crucial role in the cellular response to amino acid availability through regulation of the mTORC1 signaling cascade. Forms heterodimeric Rag complexes with RRAGC or RRAGD and cycles between an inactive GDP-bound and an active GTP-bound form. In its active form participates in the relocalization of mTORC1 to the lysosomes and its subsequent activation by the GTPase RHEB. Involved in the RCC1/Ran-GTPase pathway.
Subunit Structure: Interacts with RRAGC and RRAGD; heterodimerization stabilizes RRAG proteins (PubMed:11073942). In complex with RRAGC, but not with RRAGA, interacts with RPTOR; this interaction is particularly efficient with GTP-loaded RRAGB and GDP-loaded RRAGC (PubMed:18497260). Interacts with SH3BP4; the interaction with this negative regulator is most probably direct, preferentially occurs with the inactive GDP-bound form of RRAGB, is negatively regulated by amino acids and prevents interaction with RPTOR (PubMed:22575674). Interacts with the GATOR1 complex; inactivates RRAGB (PubMed:23723238). The Rag heterodimer interacts with SLC38A9; the probable amino acid sensor (PubMed:25561175, PubMed:25567906). Interacts with SESN1, SESN2 AND SESN3 (PubMed:25259925).
Similarity: Belongs to the GTR/RAG GTP-binding protein family.

NCBI and Uniprot Product Information

• NCBI GI #: 46249395
• NCBI GeneID: 10325
• NCBI Accession #: NP_006055.3
• NCBI GenBank Nucleotide #: NM_006064.4
• UniProt Accession #: Q5VZM2; Q15348; Q8N3T0; A8K042
• Molecular Weight: Observed: 43 kD; Predicted: 44 kDa
• NCBI Official Full Name: ras-related GTP-binding protein B short isoform
• NCBI Official Synonym Full Names: Ras related GTP binding B
• NCBI Official Symbol: RRAGB
• NCBI Official Synonym Symbols: RAGB; bA465E19.1
• NCBI Protein Information: ras-related GTP-binding protein B
• UniProt Protein Name: Ras-related GTP-binding protein B
• Protein Family: Ras-related GTP-binding protein
• UniProt Gene Name: RRAGB
• UniProt Synonym Gene Names: RagB1 PublicationManual assertion based on opinion iniRef.1

NCBI Description

Ras-homologous GTPases constitute a large family of signal transducers that alternate between an activated, GTP-binding state and an inactivated, GDP-binding state. These proteins represent cellular switches that are operated by GTP-exchange factors and factors that stimulate their intrinsic GTPase activity. All GTPases of the Ras superfamily have in common the presence of six conserved motifs involved in GTP/GDP binding, three of which are phosphate-/magnesium-binding sites (PM1-PM3) and three of which are guanine nucleotide-binding sites (G1-G3). Transcript variants encoding distinct isoforms have been identified. [provided by RefSeq, Jul 2008]

Uniprot Description

Guanine nucleotide-binding protein that plays a crucial role in the cellular response to amino acid availability through regulation of the mTORC1 signaling cascade. Forms heterodimeric Rag complexes with RRAGC or RRAGD and cycles between an inactive GDP-bound and an active GTP-bound form. In its active form participates in the relocalization of mTORC1 to the lysosomes and its subsequent activation by the GTPase RHEB. Involved in the RCC1/Ran-GTPase pathway.