Rabbit RRAGA/B Polyclonal Antibody | anti-RRAGA antibody

RRAGA/B Antibody

Cat. Num. AAA9605846

• Gene Names: RRAGA; FIP1; RAGA; FIP-1
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunofluorescence, Immunocytochemistry, ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
• Synonyms: RRAGA/B; Polyclonal Antibody; RRAGA/B Antibody; Adenovirus E3 14.7 kDa-interacting protein 1; FIP-1; Rag A; RagA; Ras-related GTP-binding protein A; RRAGA; RRAGA_HUMAN; FIP1; RAg B; RAGB; Ras related GTP binding protein A; Ras-related GTP binding protein B; anti-RRAGA antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: RRAGA/B antibody detects endogenous levels of total RRAGA/B
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
• Form/Format: Liquid; Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 313

• Applicable Applications for anti-RRAGA antibody: Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA)
• Application Notes: WB: 1:500-1:1000; IF/ICC: 1:100-1:500
• Immunogen: A synthesized peptide
• Subcellular Location: Cytoplasm. Nucleus. Lysosome. Predominantly Cytoplasmic. May shuttle between the cytoplasm and nucleus, depending on the bound nucleotide state. Colocalizes in vivo with adenovirus E3-14.7K mainly to the cytoplasm especially near the nuclear membrane and in discrete foci on or near the plasma membrane.
• Tissue Specificity: Ubiquitously expressed with highest levels of expression in skeletal muscle, heart, and brain.
• Predicted Cross Reactivity: Pig, Zebrafish, Bovine, Sheep, Rabbit, Chicken, Xenopus
• Similarity: Pig (100%), Zebrafish (90%), Bovine (100%), Sheep (100%), Rabbit (100%), Chicken (100%), Xenopus (100%)
• Conjugation: Unconjugated
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Western blot analysis of extracts from HepG2 cells, using RRAGA/B antibody.)

Immunofluorescene (IF)

(MBS9605846 staining HepG2 cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)

Related Product Information for anti-RRAGA antibody

Function: Guanine nucleotide-binding protein that plays a crucial role in the cellular response to amino acid availability through regulation of the mTORC1 signaling cascade. Forms heterodimeric Rag complexes with RRAGC or RRAGD and cycles between an inactive GDP-bound and an active GTP-bound form. In its active form participates in the relocalization of mTORC1 to the lysosomes and its subsequent activation by the GTPase RHEB. Involved in the RCC1/Ran-GTPase pathway. May play a direct role in a TNF-alpha signaling pathway leading to induction of cell death. May alternatively act as a cellular target for adenovirus E3-14.7K, an inhibitor of TNF-alpha functions, thereby affecting cell death.
Subunit Structure: Can occur as a homodimer or as a heterodimer with RRAGC or RRAGD in a sequence-independent manner; heterodimerization stabilizes proteins of the heterodimer (PubMed:11073942, PubMed:20381137). In complex with RRAGC, but not with RRAGB, interacts with RPTOR (PubMed:18497260). The GTP-bound form of RRAGA interacts with NOL8 (PubMed:14660641). Interacts with SH3BP4; the interaction with this negative regulator is most probably direct, preferentially occurs with the inactive GDP-bound form of RRAGA and is negatively regulated by amino acids (PubMed:22575674). The Rag heterodimer interacts with SLC38A9; the probable amino acid sensor (PubMed:25561175, PubMed:25567906). Interacts (inactive GDP-bound form) with RNF152; stimulated by amino acid starvation (PubMed:25936802). Interacts (polyubiquitinated) with the GATOR1 complex; inactivates RRAGA (PubMed:25936802). Interacts (polyubiquitinated) with TSC2 (PubMed:25936802). Interacts with adenovirus E3 14.7 kDa protein (PubMed:8995684). Interacts with SESN1, SESN2 AND SESN3 (PubMed:25259925).
Post-translational Modifications: Ubiquitinated. 'Lys-68'-linked polyubiquitination of the GDP-bound inactive form of RRAGA by RNF152 is increased in response to amino acid starvation. Polyubiquitination promotes interaction with the GATOR1 complex. This does not affect RRAGA degradation.
Similarity: Belongs to the GTR/RAG GTP-binding protein family.

NCBI and Uniprot Product Information

• NCBI GI #: 5729999
• NCBI GeneID: 10670
• NCBI Accession #: NP_006561.1
• NCBI GenBank Nucleotide #: NM_006570.4
• UniProt Accession #: Q7L523; O00290; Q15347; B2R7L1
• Molecular Weight: Observed: 34 kD; Predicted: 37 kDa
• NCBI Official Full Name: ras-related GTP-binding protein A
• NCBI Official Synonym Full Names: Ras related GTP binding A
• NCBI Official Symbol: RRAGA
• NCBI Official Synonym Symbols: FIP1; RAGA; FIP-1
• NCBI Protein Information: ras-related GTP-binding protein A
• UniProt Protein Name: Ras-related GTP-binding protein A
• UniProt Gene Name: RRAGA
• UniProt Synonym Gene Names: Rag A1 PublicationManual assertion based on opinion iniRef.8; RagA1 PublicationManual assertion based on opinion iniRef.1

Uniprot Description

Guanine nucleotide-binding protein that plays a crucial role in the cellular response to amino acid availability through regulation of the mTORC1 signaling cascade. Forms heterodimeric Rag complexes with RRAGC or RRAGD and cycles between an inactive GDP-bound and an active GTP-bound form. In its active form participates in the relocalization of mTORC1 to the lysosomes and its subsequent activation by the GTPase RHEB. Involved in the RCC1/Ran-GTPase pathway. May play a direct role in a TNF-alpha signaling pathway leading to induction of cell death. May alternatively act as a cellular target for adenovirus E3-14.7K, an inhibitor of TNF-alpha functions, thereby affecting cell death.