Rabbit anti-Human RRAGB Polyclonal Antibody | anti-RRAGB antibody

RRAGB, NT (RRAGB, Ras-related GTP-binding protein B) (FITC)

Cat. Num. AAA6344032

• Gene Names: RRAGB; RAGB; bA465E19.1
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: RRAGB; Polyclonal Antibody; NT (RRAGB; Ras-related GTP-binding protein B) (FITC); Ras-related GTP-binding protein B; anti-RRAGB antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-RRAGB antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: RRAGB antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human RRAGB.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(RRAGB Antibody (N-term) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the RRAGB antibody detected the RRAGB protein (arrow).)

Related Product Information for anti-RRAGB antibody

Ras-homologous GTPases constitute a large family of signal transducers that alternate between an activated, GTP-binding state and an inactivated, GDP-binding state. These proteins represent cellular switches that are operated by GTP-exchange factors and factors that stimulate their intrinsic GTPase activity. All GTPases of the Ras superfamily have in common the presence of six conserved motifs involved in GTP/GDP binding, three of which are phosphate-/magnesium-binding sites (PM1-PM3) and three of which are guanine nucleotide-binding sites (G1-G3). Transcript variants encoding distinct isoforms have been identified. [provided by RefSeq].

Product Categories/Family for anti-RRAGB antibody

Antibodies; GTPase; Rab; Ras; Rho Proteins

References

Ross, M.T., et al. Nature 434(7031):325-337(2005). Tomarev, S.I., et al. Invest. Ophthalmol. Vis. Sci. 44(6):2588-2596(2003). Sekiguchi, T., et al. J. Biol. Chem. 276(10):7246-7257(2001). Hirose, E., et al. J. Cell. Sci. 111 (PT 1), 11-21 (1998): Schurmann, A., et al. J. Biol. Chem. 270(48):28982-28988(1995).

NCBI and Uniprot Product Information

• NCBI GI #: 46249395
• NCBI GeneID: 10325
• NCBI Accession #: NP_006055.3
• NCBI GenBank Nucleotide #: NM_006064.4
• UniProt Accession #: Q5VZM2; Q15348; Q8N3T0; A8K042
• Molecular Weight: 43,250 Da
• NCBI Official Full Name: ras-related GTP-binding protein B short isoform
• NCBI Official Synonym Full Names: Ras-related GTP binding B
• NCBI Official Symbol: RRAGB
• NCBI Official Synonym Symbols: RAGB; bA465E19.1
• NCBI Protein Information: ras-related GTP-binding protein B; rag B; GTP-binding protein ragB
• UniProt Protein Name: Ras-related GTP-binding protein B
• Protein Family: Ras-related GTP-binding protein
• UniProt Gene Name: RRAGB
• UniProt Synonym Gene Names: Rag B; RagB
• UniProt Entry Name: RRAGB_HUMAN

NCBI Description

Ras-homologous GTPases constitute a large family of signal transducers that alternate between an activated, GTP-binding state and an inactivated, GDP-binding state. These proteins represent cellular switches that are operated by GTP-exchange factors and factors that stimulate their intrinsic GTPase activity. All GTPases of the Ras superfamily have in common the presence of six conserved motifs involved in GTP/GDP binding, three of which are phosphate-/magnesium-binding sites (PM1-PM3) and three of which are guanine nucleotide-binding sites (G1-G3). Transcript variants encoding distinct isoforms have been identified. [provided by RefSeq, Jul 2008]

Uniprot Description

RRAGB: Has guanine nucleotide-binding activity but undetectable intrinsic GTPase activity. Required for the amino acid-induced relocalization of mTORC1 to the lysosomes and its subsequent activation by the GTPase RHEB. This is a crucial step in the activation of the TOR signaling cascade by amino acids. Involved in the RCC1/Ran-GTPase pathway. Involved in the RCC1/Ran-GTPase pathway. The short isoform binds GTP. Interacts with RRAGC and RRAGD. Belongs to the GTR/RAG GTP-binding protein family. 2 isoforms of the human protein are produced by alternative splicing.

Chromosomal Location of Human Ortholog: Xp11.21

Cellular Component: Golgi apparatus; intracellular membrane-bound organelle; lysosomal membrane; lysosome; cytoplasm; nucleus

Molecular Function: GTPase activity; protein binding; GDP binding; GTP binding; guanyl ribonucleotide binding

Biological Process: metabolic process; regulation of autophagy; cellular response to starvation; positive regulation of TOR signaling pathway