Rabbit anti-Mouse RPS6KC1 Polyclonal Antibody | anti-RPS6KC1 antibody

RPS6KC1, NT (RPS6KC1, RPK118, Ribosomal protein S6 kinase delta-1, 52kD ribosomal protein S6 kinase, Ribosomal S6 kinase-like protein with two PSK domains 118kD protein, SPHK1-binding protein)

Cat. Num. AAA647893

• Gene Names: RPS6KC1; RPK118; humS6PKh1
• Reactivity: Mouse
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: RPS6KC1; Polyclonal Antibody; NT (RPS6KC1; RPK118; Ribosomal protein S6 kinase delta-1; 52kD ribosomal protein S6 kinase; Ribosomal S6 kinase-like protein with two PSK domains 118kD protein; SPHK1-binding protein); Anti -RPS6KC1; anti-RPS6KC1 antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Mouse
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.

• Applicable Applications for anti-RPS6KC1 antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in Western Blot, Immunohistochemistry, ELISA; Dilution: ELISA: 1:1,000; Western Blot: 1:100-500; Immunohistochemistry: 1:10-50
• Immunogen: RPS6KC1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 19-47 amino acids from the N-terminal region of human RPS6KC1.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(RPS6KC1 Antibody (N-term) western blot analysis in mouse liver tissue lysates (35ug/lane).This demonstrates the RPS6KC1 antibody detected the RPS6KC1 protein (arrow).)

Immunohistochemistry (IHC)

(RPS6KC1 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of RPS6KC1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Related Product Information for anti-RPS6KC1 antibody

RPS6KC1 may be involved in transmitting sphingosine-1 phosphate (SPP)-mediated signaling into the cell.

Product Categories/Family for anti-RPS6KC1 antibody

Antibodies; Abs to Protein Kinases

NCBI and Uniprot Product Information

• NCBI GI #: 209915591
• NCBI GeneID: 26750
• NCBI Accession #: NP_001129610.1
• NCBI GenBank Nucleotide #: NM_001136138.1
• UniProt Accession #: Q96S38; Q8TDD3; Q9NSF4; Q9UL66; B1APS8; B3KVM4; D3DTA4
• Molecular Weight: 118,682 Da
• NCBI Official Full Name: ribosomal protein S6 kinase delta-1 isoform b
• NCBI Official Synonym Full Names: ribosomal protein S6 kinase, 52kDa, polypeptide 1
• NCBI Official Symbol: RPS6KC1
• NCBI Official Synonym Symbols: RPK118; humS6PKh1
• NCBI Protein Information: ribosomal protein S6 kinase delta-1; S6K-delta-1; SPHK1-binding protein; 52 kDa ribosomal protein S6 kinase; ribosomal S6 kinase-like protein with two PSK domains 118 kDa protein
• UniProt Protein Name: Ribosomal protein S6 kinase delta-1
• Protein Family: Ribosomal protein S6 kinase
• UniProt Gene Name: RPS6KC1
• UniProt Synonym Gene Names: RPK118; S6K-delta-1
• UniProt Entry Name: KS6C1_HUMAN

Uniprot Description

Function: May be involved in transmitting sphingosine-1 phosphate (SPP)-mediated signaling into the cell.

Catalytic activity: ATP + a protein = ADP + a phosphoprotein.

Subunit structure: Interacts with SPHK1 and phosphatidylinositol 3-phosphate. Ref.1

Subcellular location: Cytoplasm. Membrane. Note: Also found in some small dot-like or ring-shaped early endosome structures. Ref.1

Tissue specificity: Highly expressed in testis, skeletal muscle, brain, heart, placenta, kidney and liver and weakly expressed in thymus, small intestine, lung and colon. Ref.1

Domain: The first protein kinase domain appears to be a pseudokinase domain as it does not contain the classical characteristics, such as the ATP-binding motif, ATP-binding site and active site.

Sequence similarities: Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. S6 kinase subfamily.Contains 1 MIT domain.Contains 2 protein kinase domains.Contains 1 PX (phox homology) domain.

Caution: Instead of Lys-820, Arg-820 is found at the binding site.

Sequence caution: The sequence CAB92850.1 differs from that shown. Reason: Erroneous initiation.