Rabbit anti-Human AARS Polyclonal Antibody | anti-AARS antibody

AARS (Alanyl-tRNA Synthetase, Alanine-tRNA Ligase, Cytoplasmic, AlaRS, Renal Carcinoma Antigen NY-REN-42) (HRP)

Cat. Num. AAA6368377

• Gene Names: AARS; CMT2N
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: AARS; Polyclonal Antibody; AARS (Alanyl-tRNA Synthetase; Alanine-tRNA Ligase; Cytoplasmic; AlaRS; Renal Carcinoma Antigen NY-REN-42) (HRP); anti-AARS antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human AARS.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-AARS antibody: Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: Full length human AARS, aa1-968 (NP_001596.2).
• Immunogen Sequence: MDSTLTASEIRQRFIDFFKRNEHTYVHSSATIPLDDPTLLFANAGMNQFKPIFLNTIDPSHPMAKLSRAANTQKCIRAGGKHNDLDDVGKDVYHHTFFEMLGSWSFGDYFKELACKMALELLTQEFGIPIERLYVTYFGGDEAAGLEADLECKQIWQNLGLDDTKILPGNMKDNFWEMGDTGPCGPCSEIHYDRIGGRDAAHLVNQDDPNVLEIWNLVFIQYNREADGILKPLPKKSIDTGMGLERLVSVLQNKMSNYDTDLFVPYFEAIQKGTGARPYTGKVGAEDADGIDMAYRVLADHARTITVALADGGRPDNTGRGYVLRRILRRAVRYAHEKLNASRGFFATLVDVVVQSLGDAFPELKKDPDMVKDIINEEEVQFLKTLSRGRRILDRKIQSLGDSKTIPGDTAWLLYDTYGFPVDLTGLIAEEKGLVVDMDGFEEERKLAQLKSQGKGAGGEDLIMLDIYAIEELRARGLEVTDDSPKYNYHLDSSGSYVFENTVATVMALRREKMFVEEVSTGQECGVVLDKTCFYAEQGGQIYDEGYLVKVDDSSEDKTEFTVKNAQVRGGYVLHIGTIYGDLKVGDQVWLFIDEPRRRPIMSNHTATHILNFALRSVLGEADQKGSLVAPDRLRFDFTAKGAMSTQQIKKAEEIANEMIEAAKAVYTQDCPLAAAKAIQGLRAVFDETYPDPVRVVSIGVPVSELLDDPSGPAGSLTSVEFCGGTHLRNSSHAGAFVIVTEEAIAKGIRRIVAVTGAEAQKALRKAESLKKCLSVMEAKVKAQTAPNKDVQREIADLGEALATAVIPQWQKDELRETLKSLKKVMDDLDRASKADVQKRVLEKTKQFIDSNPNQPLVILEMESGASAKALNEALKLFKMHSPQTSAMLFTVDNEAGKITCLCQVPQNAANRGLKASEWVQQVSGLMDGKGGGKDVSAQATGKNVGCLQEALQLATSFAQLRLGDVKN
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(AARS rabbit polyclonal antibody. Western Blot analysis of AARS expression in human kidney.)

Western Blot (WB)

(AARS rabbit polyclonal antibody. Western Blot analysis of AARS expression in Jurkat.)

Western Blot (WB)

(Western Blot analysis of AARS expression in transfected 293T cell line by AARS polyclonal antibody. Lane 1: AARS transfected lysate (106.8kD). Lane 2: Non-transfected lysate.)

Related Product Information for anti-AARS antibody

The human alanyl-tRNA synthetase (AARS) belongs to a family of tRNA synthases,of the class II enzymes. Class II tRNA synthases evolved early in evolution and are highly conserved.This is reflected by the fact that 498 of the 968-residue polypeptide human AARS shares 41% identity witht the E.coli protein. tRNA synthases are the enzymes that interpret the RNA code and attach specific aa to the tRNAs that contain the cognate trinucleotide anticodons.They consist of a catalytic domain which interacts with the aa acceptor-T psi C helix of the tRNA, and a second domain which interacts with the rest of the tRNA structure.

Product Categories/Family for anti-AARS antibody

Antibodies; Enzymes; Synthase

NCBI and Uniprot Product Information

• NCBI GI #: 109148542
• NCBI GeneID: 16
• NCBI Accession #: NP_001596.2
• NCBI GenBank Nucleotide #: NM_001605.2
• UniProt Accession #: P49588; Q53GV7; Q96FA0; A6NF14; B4DR45
• Molecular Weight: 109,317 Da
• NCBI Official Full Name: alanine--tRNA ligase, cytoplasmic
• NCBI Official Synonym Full Names: alanyl-tRNA synthetase
• NCBI Official Symbol: AARS
• NCBI Official Synonym Symbols: CMT2N
• NCBI Protein Information: alanine--tRNA ligase, cytoplasmic; AARS; alaRS; alanine tRNA ligase 1, cytoplasmic; alanyl-tRNA synthetase, cytoplasmic; renal carcinoma antigen NY-REN-42
• UniProt Protein Name: Alanine--tRNA ligase, cytoplasmic
• UniProt Gene Name: AARS
• UniProt Entry Name: SYAC_HUMAN

NCBI Description

The human alanyl-tRNA synthetase (AARS) belongs to a family of tRNA synthases, of the class II enzymes. Class II tRNA synthases evolved early in evolution and are highly conserved. This is reflected by the fact that 498 of the 968-residue polypeptide human AARS shares 41% identity witht the E.coli protein. tRNA synthases are the enzymes that interpret the RNA code and attach specific aminoacids to the tRNAs that contain the cognate trinucleotide anticodons. They consist of a catalytic domain which interacts with the amino acid acceptor-T psi C helix of the tRNA, and a second domain which interacts with the rest of the tRNA structure. [provided by RefSeq, Jul 2008]

Uniprot Description

AARS: Catalyzes the attachment of alanine to tRNA(Ala) in a two-step reaction: alanine is first activated by ATP to form Ala- AMP and then transferred to the acceptor end of tRNA(Ala). Also edits incorrectly charged tRNA(Ala) via its editing domain. Defects in AARS are the cause of Charcot-Marie-Tooth disease type 2N (CMT2N). It is an axonal form of Charcot-Marie-Tooth disease, a disorder of the peripheral nervous system, characterized by progressive weakness and atrophy, initially of the peroneal muscles and later of the distal muscles of the arms. Charcot-Marie-Tooth disease is classified in two main groups on the basis of electrophysiologic properties and histopathology: primary peripheral demyelinating neuropathies(designated CMT1 when they are dominantly inherited) and primary peripheral axonal neuropathies (CMT2). Neuropathies of the CMT2 group are characterized by signs of axonal regeneration in the absence of obvious myelin alterations, normal or slightly reduced nerve conduction velocities, and progressive distal muscle weakness and atrophy. Nerve conduction velocities are normal or slightly reduced. Belongs to the class-II aminoacyl-tRNA synthetase family.

Protein type: RNA-binding; Ligase; EC 6.1.1.7; Translation

Chromosomal Location of Human Ortholog: 16q22

Cellular Component: membrane; cytoplasm; cytosol

Molecular Function: amino acid binding; zinc ion binding; ATP binding; alanine-tRNA ligase activity; tRNA binding

Biological Process: protein folding; unfolded protein response; tRNA processing; regulation of translational fidelity; cerebellar Purkinje cell layer development; tRNA modification; response to amino acid stimulus; tRNA aminoacylation for protein translation; hair follicle development; gene expression; negative regulation of neuron apoptosis; neuromuscular process controlling balance; alanyl-tRNA aminoacylation

Disease: Epileptic Encephalopathy, Early Infantile, 29; Charcot-marie-tooth Disease, Axonal, Type 2n