Meprin alpha/MEP1A Recombinant Protein | MEP1A recombinant protein

Meprin alpha/MEP1A Protein, Human, Recombinant (His Tag)

Cat. Num. AAA8120016

• Gene Names: MEP1A; PPHA
• Purity: >90% as determined by SDS-PAGE
• Synonyms: Meprin alpha/MEP1A; Meprin alpha/MEP1A Protein; Human; Recombinant (His Tag); Human MEP1A/PPHA Protein (His Tag); PPHA Protein; meprin A; alpha (PABA peptide hydrolase); MEP1A recombinant protein

• Host: HEK293 Cells
• Purity/Purification: >90% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Gln601

• Species: Human
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Val 22
• Tag: C-His
• Protein Construction: A DNA sequence encoding the human MEP1A (NP_005579.2) extracellular domain (Met 1-Gln 601) was expressed, fused with a polyhistidine tag at the C-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for MEP1A recombinant protein

Background: Meprin A subunit alpha, also known as MEP1A, and Endopeptidase-2, is a single-pass type I membrane protein that belongs to the peptidase M12A family. MEP1A contains one EGF-like domain, one MAM domain, and one MATH domain. Meprins are unique plasma membrane and secreted metalloproteinases that are highly regulated at the transcriptional and post-translational levels. Meprin alpha and beta subunits are abundantly expressed in kidney and intestinal epithelial cells, are secreted into the urinary tract and intestinal lumen and are found in leukocytes and cancer cells under certain conditions. Meprins are capable of proteolytically degrading extracellular matrix proteins, processing bioactive proteins, and play a role in inflammatory processes. Meprin A and B are highly regulated, secreted and cell-surface homo- and hetero-oligomeric enzymes. Meprins are abundantly expressed in the kidney and intestine. The multidomain alpha and beta subunits have high sequence identity. They have very different substrate specificities, oligomerization potentials, and are differentially regulated. Meprin A appears to be an important therapeutic target and urinary excretion appears to be a potential biomarker of acute kidney injury (AKI).

References

Bertenshaw,GP. et al., 2002, Biol Chem. 383 (7-8):1175-83. Bond, JS. et al., 2005, FEBS Lett. 579 (15): 3317-22. Herzog, C. et al., 2007, Kidney Int. 71 (10): 1009-18. Yura, RE. et al., 2009, Am J Physiol Renal Physiol. 296 (1): F135-44.

NCBI and Uniprot Product Information

• NCBI GI #: 153070262
• NCBI GeneID: 4224
• NCBI Accession #: NP_005579.2
• NCBI GenBank Nucleotide #: NM_005588.2
• UniProt Accession #: Q16819; Q8TDC9; Q9H1R1; A2RRM4; B0AZP9; B2RCS2
• Molecular Weight: 84,419 Da
• NCBI Official Full Name: meprin A subunit alpha
• NCBI Official Synonym Full Names: meprin A subunit alpha
• NCBI Official Symbol: MEP1A
• NCBI Official Synonym Symbols: PPHA
• NCBI Protein Information: meprin A subunit alpha
• UniProt Protein Name: Meprin A subunit alpha
• Protein Family: Meprin
• UniProt Gene Name: MEP1A

Uniprot Description

MEP1A: Belongs to the peptidase M12A family.

Protein type: EC 3.4.24.18; Membrane protein, integral; Protease

Chromosomal Location of Human Ortholog: 6p12.3

Cellular Component: extracellular space; integral to plasma membrane

Molecular Function: protein binding

Biological Process: digestion