Rabbit anti-Human RPS6KA5 Polyclonal Antibody | anti-RPS6KA5 antibody

RPS6KA5 Antibody (C-term)

Cat. Num. AAA9204584

• Gene Names: RPS6KA5; MSK1; RLPK; MSPK1
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified Rabbit Polyclonal Antibody (Pab)
• Synonyms: RPS6KA5; Polyclonal Antibody; RPS6KA5 Antibody (C-term); Ribosomal protein S6 kinase alpha-5; S6K-alpha-5; 90 kDa ribosomal protein S6 kinase 5; Nuclear mitogen- and stress-activated protein kinase 1; RSK-like protein kinase; RSKL; MSK1; anti-RPS6KA5 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Specificity: This RPS6KA5 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 731-765 amino acids from the C-terminal region of human RPS6KA5.
• Purity/Purification: Purified Rabbit Polyclonal Antibody (Pab)
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Concentration: Vial Concentration: 0.5 (varies by lot)
• Sequence Length: 723

• Applicable Applications for anti-RPS6KA5 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB~~1:4000
• Antigen Source: HUMAN
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(Anti-RPS6KA5 Antibody (C-term) at 1:4000 dilution + Hela whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 90 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Related Product Information for anti-RPS6KA5 antibody

Serine/threonine-protein kinase that is required for the mitogen or stress-induced phosphorylation of the transcription factors CREB1 and ATF1 and for the regulation of the transcription factors RELA, STAT3 and ETV1/ER81, and that contributes to gene activation by histone phosphorylation and functions in the regulation of inflammatory genes. Phosphorylates CREB1 and ATF1 in response to mitogenic or stress stimuli such as UV-C irradiation, epidermal growth factor (EGF) and anisomycin. Plays an essential role in the control of RELA transcriptional activity in response to TNF and upon glucocorticoid, associates in the cytoplasm with the glucocorticoid receptor NR3C1 and contributes to RELA inhibition and repression of inflammatory gene expression. In skeletal myoblasts is required for phosphorylation of RELA at 'Ser-276' during oxidative stress. In erythropoietin-stimulated cells, is necessary for the 'Ser-727' phosphorylation of STAT3 and regulation of its transcriptional potential. Phosphorylates ETV1/ER81 at 'Ser-191' and 'Ser-216', and thereby regulates its ability to stimulate transcription, which may be important during development and breast tumor formation. Directly represses transcription via phosphorylation of 'Ser-1' of histone H2A. Phosphorylates 'Ser-10' of histone H3 in response to mitogenics, stress stimuli and EGF, which results in the transcriptional activation of several immediate early genes, including proto- oncogenes c-fos/FOS and c-jun/JUN. May also phosphorylate 'Ser-28' of histone H3. Mediates the mitogen- and stress-induced phosphorylation of high mobility group protein 1 (HMGN1/HMG14). In lipopolysaccharide-stimulated primary macrophages, acts downstream of the Toll-like receptor TLR4 to limit the production of pro- inflammatory cytokines. Functions probably by inducing transcription of the MAP kinase phosphatase DUSP1 and the anti- inflammatory cytokine interleukin 10 (IL10), via CREB1 and ATF1 transcription factors. Plays a role in neuronal cell death by mediating the downstream effects of excitotoxic injury.

References

Deak M.,et al.EMBO J. 17:4426-4441(1998).
New L.,et al.J. Biol. Chem. 274:1026-1032(1999).
Jiang C.,et al.Cytogenet. Cell Genet. 87:261-262(1999).
Heilig R.,et al.Nature 421:601-607(2003).
Wiggin G.R.,et al.Mol. Cell. Biol. 22:2871-2881(2002).

NCBI and Uniprot Product Information

• NCBI GI #: 32528295
• NCBI GeneID: 9252
• NCBI Accession #: NP_004746.2
• NCBI GenBank Nucleotide #: NM_004755.2
• UniProt Accession #: O75582; O95316; Q96AF7; B7Z2Y5
• Molecular Weight: 89865
• NCBI Official Full Name: ribosomal protein S6 kinase alpha-5 isoform a
• NCBI Official Synonym Full Names: ribosomal protein S6 kinase, 90kDa, polypeptide 5
• NCBI Official Symbol: RPS6KA5
• NCBI Official Synonym Symbols: MSK1; RLPK; MSPK1
• NCBI Protein Information: ribosomal protein S6 kinase alpha-5
• UniProt Protein Name: Ribosomal protein S6 kinase alpha-5
• Protein Family: Ribosomal protein S6 kinase
• UniProt Gene Name: RPS6KA5
• UniProt Synonym Gene Names: MSK1; S6K-alpha-5; RSKL
• UniProt Entry Name: KS6A5_HUMAN

Uniprot Description

MSK1: an AGC kinase of the RSK family. Has two kinase domains connected by a regulatory linker region. Enzyme activity requires the presence of both kinase domains. Is phosphorylated and activated by Erk as well as p38 MAPK in response to growth factors and cellular stress.

Protein type: Protein kinase, AGC; Protein kinase, Ser/Thr (non-receptor); Kinase, protein; EC 2.7.11.1; AGC group; RSK family; MSK subfamily

Chromosomal Location of Human Ortholog: 14q31-q32.1

Cellular Component: nucleoplasm; cytoplasm; nucleus

Molecular Function: protein serine/threonine kinase activity; protein binding; magnesium ion binding; ATP binding; protein kinase activity

Biological Process: epidermal growth factor receptor signaling pathway; axon guidance; nerve growth factor receptor signaling pathway; positive regulation of histone phosphorylation; histone phosphorylation; MyD88-independent toll-like receptor signaling pathway; activation of CREB transcription factor; stress-activated MAPK cascade; toll-like receptor 3 signaling pathway; protein amino acid phosphorylation; toll-like receptor 10 signaling pathway; toll-like receptor 2 signaling pathway; activation of NF-kappaB transcription factor; toll-like receptor 5 signaling pathway; MyD88-dependent toll-like receptor signaling pathway; regulation of transcription, DNA-dependent; negative regulation of cytokine production; toll-like receptor signaling pathway; innate immune response; positive regulation of transcription from RNA polymerase II promoter; positive regulation of histone acetylation; toll-like receptor 9 signaling pathway; negative regulation of transcription, DNA-dependent; inflammatory response; toll-like receptor 4 signaling pathway