Rabbit anti-Human CCT7 Polyclonal Antibody | anti-CCT7 antibody

CCT7 Antibody

Cat. Num. AAA7106225

• Gene Names: CCT7; CCTH; CCTETA; NIP7-1; TCP1ETA
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: >95%, Protein G purified
• Synonyms: CCT7; Polyclonal Antibody; CCT7 Antibody; T-complex protein 1 subunit eta; TCP-1-eta; CCT-eta; HIV-1 Nef-interacting protein; N-terminally processed; CCTH; NIP7-1; anti-CCT7 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: >95%, Protein G purified
• Form/Format: Liquid
• Sequence Length: 339

• Applicable Applications for anti-CCT7 antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: WB: 1:500-1:5000; IHC: 1:20-1:200; IF: 1:50-1:200
• Immunogen: Recombinant Human T-complex protein 1 subunit eta protein (240-543AA)
• Conjugation: Non-conjugated
• Buffer: Preservative: 0.03% Proclin 300; Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
• Preparation and Storage: Upon receipt, store at -20 degree C or -80 degree C. Avoid repeated freeze.

Western Blot (WB)

(Western BlotPositive WB detected in: K562 whole cell lysate, 293 whole cell lysateAll lanes: CCT7 antibody at 3ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 60, 38, 55, 51 KDaObserved band size: 60 KDa)

Immunohistochemistry (IHC)

(IHC image of MBS7106225 diluted at 1:100 and staining in paraffin-embedded human glioma cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)

Immunohistochemistry (IHC)

(Immunofluorescence staining of MCF-7 cells with MBS7106225 at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4 degree C.The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).)

Related Product Information for anti-CCT7 antibody

Molecular chaperone; assists the folding of proteins upon ATP hydrolysis. Known to play a role, in vitro, in the folding of actin and tubulin (By similarity).

Product Categories/Family for anti-CCT7 antibody

Immunology; Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 58331185
• NCBI GeneID: 10574
• NCBI Accession #: NP_001009570.1
• NCBI GenBank Nucleotide #: NM_001009570.2
• UniProt Accession #: Q99832; O14871; Q6FI26; A8K7E6; A8MWI8; B7WNW9; B7Z4T9; B7Z4Z7
• Molecular Weight: 50,352 Da
• NCBI Official Full Name: T-complex protein 1 subunit eta isoform b
• NCBI Official Synonym Full Names: chaperonin containing TCP1 subunit 7
• NCBI Official Symbol: CCT7
• NCBI Official Synonym Symbols: CCTH; CCTETA; NIP7-1; TCP1ETA
• NCBI Protein Information: T-complex protein 1 subunit eta
• UniProt Protein Name: T-complex protein 1 subunit eta
• Protein Family: Probable T-complex protein
• UniProt Gene Name: CCT7
• UniProt Synonym Gene Names: CCTH; NIP7-1; TCP-1-eta

NCBI Description

This gene encodes a molecular chaperone that is a member of the chaperonin containing TCP1 complex (CCT), also known as the TCP1 ring complex (TRiC). This complex consists of two identical stacked rings, each containing eight different proteins. Unfolded polypeptides enter the central cavity of the complex and are folded in an ATP-dependent manner. The complex folds various proteins, including actin and tubulin. Alternative splicing results in multiple transcript variants. Related pseudogenes have been identified on chromosomes 5 and 6. [provided by RefSeq, Oct 2009]

Uniprot Description

Molecular chaperone; assists the folding of proteins upon ATP hydrolysis. Known to play a role, in vitro, in the folding of actin and tubulin ().