Human Zinc finger E-box-binding homeobox 2 ELISA Kit | ZEB2 elisa kit

Human Zinc finger E-box-binding homeobox 2 ELISA Kit

Cat. Num. AAA763378

• Gene Names: ZEB2; SIP1; SIP-1; ZFHX1B; HSPC082; SMADIP1
• Reactivity: Human
• Synonyms: Zinc finger E-box-binding homeobox 2; Human Zinc finger E-box-binding homeobox 2 ELISA Kit; ZEB2/Zinc finger E-box-binding homeobox 2/Smad-interacting protein 1/SMADIP1/Zinc finger homeobox protein 1b/SIP1/SIP-1/ZFHX1B/HSPC082/HRIHFB2411/KIAA0569/ZFHX1B/ZFX1B; ZEB2 elisa kit

• Reactivity: Human
• Sequence Length: 1190

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 0.312-20ng/ml
• Sensitivity: 0.188ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for ZEB2 elisa kit

Principle of the Assay||This ELISA kit uses Competitive-ELISA as the method. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human ZEB2. During the reaction,Human ZEB2 in the sample or standard competes with a fixed amount of biotin-labeled Human ZEB2 for sites on a precoated Monoclonal antibody specific to Human ZEB2. Excess conjugate and unbound sample or standard are washed from the plate, and HRP-Streptavidin (SABC) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of Human ZEB2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 284413746
• NCBI GeneID: 9839
• NCBI Accession #: NP_001165124.1
• NCBI GenBank Nucleotide #: NM_001171653.1
• Molecular Weight: 133,806 Da
• NCBI Official Full Name: zinc finger E-box-binding homeobox 2 isoform 2
• NCBI Official Synonym Full Names: zinc finger E-box binding homeobox 2
• NCBI Official Symbol: ZEB2
• NCBI Official Synonym Symbols: SIP1; SIP-1; ZFHX1B; HSPC082; SMADIP1
• NCBI Protein Information: zinc finger E-box-binding homeobox 2
• UniProt Protein Name: Zinc finger E-box-binding homeobox 2
• Protein Family: Zinc finger E-box-binding homeobox
• UniProt Gene Name: ZEB2
• UniProt Synonym Gene Names: KIAA0569; SIP1; ZFHX1B; ZFX1B; SMADIP1
• UniProt Entry Name: ZEB2_HUMAN

NCBI Description

The protein encoded by this gene is a member of the Zfh1 family of 2-handed zinc finger/homeodomain proteins. It is located in the nucleus and functions as a DNA-binding transcriptional repressor that interacts with activated SMADs. Mutations in this gene are associated with Hirschsprung disease/Mowat-Wilson syndrome. Alternatively spliced transcript variants have been found for this gene.[provided by RefSeq, Jan 2010]

Uniprot Description

ZEB2: Transcriptional inhibitor that binds to DNA sequence 5'- CACCT-3' in different promoters. Represses transcription of E- cadherin. Defects in ZEB2 are the cause of Mowat-Wilson syndrome (MWIS); also known as Hirschsprung disease-mental retardation syndrome. A complex developmental disorder characterized by mental retardation, delayed motor development, epilepsy, microcephaly and a wide spectrum of clinically heterogeneous features suggestive of neurocristopathies at the cephalic, cardiac, and vagal levels. Some patients manifest Hirschsprung disease. Affected patients show an easily recognizable facial appearance with deep set eyes and hypertelorism, medially divergent, broad eyebrows, prominent columella, pointed chin and uplifted, notched ear lobes. Belongs to the delta-EF1/ZFH-1 C2H2-type zinc-finger family.

Protein type: Transcription, coactivator/corepressor; Motility/polarity/chemotaxis; DNA-binding; C2H2-type zinc finger protein

Chromosomal Location of Human Ortholog: 2q22.3

Cellular Component: nucleus

Molecular Function: DNA binding; metal ion binding; phosphatase regulator activity; protein binding

Biological Process: cell proliferation in forebrain; hippocampus development; negative regulation of transcription from RNA polymerase II promoter; nervous system development; neural crest cell migration; neural tube closure; pigmentation during development; positive regulation of JNK activity; positive regulation of melanin biosynthetic process; positive regulation of melanocyte differentiation; positive regulation of transcription from RNA polymerase II promoter; positive regulation of Wnt receptor signaling pathway; somitogenesis; transcription, DNA-dependent

Disease: Mowat-wilson Syndrome