Rabbit anti-Human RPS3A Polyclonal Antibody | anti-RPS3A antibody

RPS3A, CT (RPS3A, FTE1, MFTL, 40S ribosomal protein S3a, v-fos transformation effector protein) (Azide free) (HRP)

Cat. Num. AAA6343857

• Gene Names: RPS3A; S3A; FTE1; MFTL
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: RPS3A; Polyclonal Antibody; CT (RPS3A; FTE1; MFTL; 40S ribosomal protein S3a; v-fos transformation effector protein) (Azide free) (HRP); v-fos transformation effector protein; anti-RPS3A antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-RPS3A antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:50-100; ELISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: RPS3A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 235-264 amino acids from the C-terminal region of human RPS3A.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(RPS3A Antibody (C-term) western blot analysis in Hela,A2058 cell line lysates (15ug/lane).This demonstrates the RPS3A antibody detected the RPS3A protein (arrow).)

Immunohistochemistry (IHC)

(RPS3A Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the RPS3A Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(RPS3A Antibody (C-term) flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-RPS3A antibody

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 40S subunit. The protein belongs to the S3AE family of ribosomal proteins. It is located in the cytoplasm. Disruption of the gene encoding rat ribosomal protein S3a, also named v-fos transformation effector protein, in v-fos-transformed rat cells results in reversion of the transformed phenotype. Transcript variants utilizing alternative transcription start sites have been described. This gene is co-transcribed with the U73A and U73B small nucleolar RNA genes, which are located in its fourth and third introns, respectively. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.

Product Categories/Family for anti-RPS3A antibody

Antibodies; Ribosomal Proteins

References

Maggi, L.B. Jr., et al. Mol. Cell. Biol. 28(23):7050-7065(2008). Sugiyama, N., et al. Mol. Cell Proteomics 6(6):1103-1109(2007). Jonson, L., et al. Mol. Cell Proteomics 6(5):798-811(2007). Ewing, R.M., et al. Mol. Syst. Biol. 3, 89 (2007).

NCBI and Uniprot Product Information

• NCBI GI #: 4506723
• NCBI GeneID: 6189
• NCBI Accession #: NP_000997.1
• NCBI GenBank Nucleotide #: NM_001006.4
• UniProt Accession #: P61247; P33443; P49241; B2R4D4; D3DP05
• Molecular Weight: 29,945 Da
• NCBI Official Full Name: 40S ribosomal protein S3a isoform 1
• NCBI Official Synonym Full Names: ribosomal protein S3A
• NCBI Official Symbol: RPS3A
• NCBI Official Synonym Symbols: S3A; FTE1; MFTL
• NCBI Protein Information: 40S ribosomal protein S3a; RPS3A; fte-1; v-fos transformation effector protein 1
• UniProt Protein Name: 40S ribosomal protein S3a
• Protein Family: 40S ribosomal protein
• UniProt Gene Name: RPS3A
• UniProt Synonym Gene Names: Fte-1
• UniProt Entry Name: RS3A_HUMAN

NCBI Description

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 40S subunit. The protein belongs to the S3AE family of ribosomal proteins. It is located in the cytoplasm. Disruption of the gene encoding rat ribosomal protein S3a, also named v-fos transformation effector protein, in v-fos-transformed rat cells results in reversion of the transformed phenotype. This gene is co-transcribed with the U73A and U73B small nucleolar RNA genes, which are located in its fourth and third introns, respectively. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, May 2012]

Uniprot Description

RPS3A: ribosomal protein S3a. Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. Belongs to the S3AE family of ribosomal proteins. Located in the cytoplasm. Also named v-fos transformation effector protein.

Protein type: Translation; Ribosomal

Chromosomal Location of Human Ortholog: 4q31.2-q31.3

Cellular Component: focal adhesion; cytoplasm; nucleolus; cytosol; ribonucleoprotein complex; nucleus

Molecular Function: protein binding; structural constituent of ribosome; RNA binding

Biological Process: SRP-dependent cotranslational protein targeting to membrane; translation; viral reproduction; viral infectious cycle; translational termination; cellular protein metabolic process; translational elongation; mRNA catabolic process, nonsense-mediated decay; translational initiation; gene expression; viral transcription; cell differentiation; negative regulation of apoptosis