Rabbit RPS3A Antibody | anti-RPS3A antibody

RPS3A Polyclonal Antibody

Cat. Num. AAA9126254

• Gene Names: RPS3A; S3A; FTE1; MFTL
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence, Immunoprecipitation
• Purity: Affinity Purification
• Synonyms: RPS3A; RPS3A Polyclonal Antibody; S3A; FTE1; MFTL; anti-RPS3A antibody

• Host: Rabbit
• Isotype: IgG
• Purity/Purification: Affinity Purification
• Form/Format: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3

• Applicable Applications for anti-RPS3A antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP)
• Application Notes: WB: 1:500 - 1:2000; IHC: 1:50 - 1:200; IF: 1:50 - 1:200; IP: 1:50 - 1:100
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-264 of human RPS3A (NP_000997.1).
• Species Reactivity/Cross-Reactivity: Human, Mouse, Rat
• Preparation and Storage: Store at -20°C. Avoid freeze/thaw cycles.

Western Blot (WB)

(Western blot analysis of extracts of various cell lines, using RPS3A antibody (MBS9126254) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 90s.)

Immunoprecipitation (IP)

(Immunoprecipitation analysis of 200ug extracts of MCF7 cells using 3ug RPS3A antibody (MBS9126254). Western blot was performed from the immunoprecipitate using RPS3A antibody (MBS9126254) at a dilitionof 1:500.)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded rat brain using RPS3A Antibody (MBS9126254) at dilution of 1:100 (40x lens).)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human liver cancer using RPS3A Antibody (MBS9126254) at dilution of 1:100 (40x lens).)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded mouse brain using RPS3A Antibody (MBS9126254) at dilution of 1:100 (40x lens).)

Immunofluorescence (IF)

(Immunofluorescence analysis of MCF-7 cells using RPS3A antibody (MBS9126254). Blue: DAPI for nuclear staining.)

Related Product Information for anti-RPS3A antibody

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 40S subunit. The protein belongs to the S3AE family of ribosomal proteins. It is located in the cytoplasm. Disruption of the gene encoding rat ribosomal protein S3a, also named v-fos transformation effector protein, in v-fos-transformed rat cells results in reversion of the transformed phenotype. This gene is co-transcribed with the U73A and U73B small nucleolar RNA genes, which are located in its fourth and third introns, respectively. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. Alternatively spliced transcript variants have been found for this gene.

Product Categories/Family for anti-RPS3A antibody

Polyclonal

NCBI and Uniprot Product Information

• NCBI GI #: 47117764
• NCBI GeneID: 6189
• UniProt Accession #: P61247; P33443; P49241; B2R4D4; D3DP05
• Molecular Weight: Observed MW: 35 kDa; Calculated MW: 29 kDa
• NCBI Official Full Name: 40S ribosomal protein S3a
• NCBI Official Synonym Full Names: ribosomal protein S3A
• NCBI Official Symbol: RPS3A
• NCBI Official Synonym Symbols: S3A; FTE1; MFTL
• NCBI Protein Information: 40S ribosomal protein S3a; RPS3A; fte-1; v-fos transformation effector protein 1
• UniProt Protein Name: 40S ribosomal protein S3a
• Protein Family: 40S ribosomal protein
• UniProt Gene Name: RPS3A
• UniProt Synonym Gene Names: Fte-1
• UniProt Entry Name: RS3A_HUMAN

NCBI Description

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 40S subunit. The protein belongs to the S3AE family of ribosomal proteins. It is located in the cytoplasm. Disruption of the gene encoding rat ribosomal protein S3a, also named v-fos transformation effector protein, in v-fos-transformed rat cells results in reversion of the transformed phenotype. This gene is co-transcribed with the U73A and U73B small nucleolar RNA genes, which are located in its fourth and third introns, respectively. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, May 2012]

Uniprot Description

RPS3A: ribosomal protein S3a. Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. Belongs to the S3AE family of ribosomal proteins. Located in the cytoplasm. Also named v-fos transformation effector protein.

Protein type: Ribosomal; Translation

Chromosomal Location of Human Ortholog: 4q31.2-q31.3

Cellular Component: focal adhesion; cytoplasm; nucleolus; nucleus; ribonucleoprotein complex; cytosol

Molecular Function: protein binding; structural constituent of ribosome; RNA binding

Biological Process: SRP-dependent cotranslational protein targeting to membrane; cellular protein metabolic process; translational elongation; viral reproduction; translation; translational initiation; mRNA catabolic process, nonsense-mediated decay; viral transcription; gene expression; translational termination; viral infectious cycle; cell differentiation; negative regulation of apoptosis