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Western Blot (WB) (Western blot analysis of SHBG in human liver tissue lysate with SHBG antibody at (A) 1 and (B) 2 μg/ml.)

Rabbit SHBG Polyclonal Antibody | anti-SHBG antibody

SHBG Antibody

Gene Names
SHBG; ABP; SBP; TEBG
Reactivity
Human, Mouse, Rat
Applications
ELISA, Western Blot, Immunofluorescence
Purity
SHBG antibody is affinity chromatography purified via peptide column.
Synonyms
SHBG; Polyclonal Antibody; SHBG Antibody; Sex hormone-binding globulin; ABP; SBP; TEBG; sex hormone-binding globulin; anti-SHBG antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
SHBG antibody is human, mouse, and rat reactive. At least three isoforms of SHBG are known to exist; this antibody will detect all three isoforms.
Purity/Purification
SHBG antibody is affinity chromatography purified via peptide column.
Form/Format
Liquid
Concentration
1 mg/mL (varies by lot)
Sequence Length
402
Applicable Applications for anti-SHBG antibody
ELISA (EIA), Western Blot (WB), Immunofluorescence (IF)
Application Notes
LIN54 antibody can be used for detection of LIN54 by Western blot at 1 - 2 mug/ml. For immunofluorescence start at 20 mug/mL.
Conjugate
Unconjugated
Immunogen
SHBG antibody was raised against a 16 amino acid peptide near the center of human SHBG.
Buffer
SHBG antibody is supplied in PBS containing 0.02% sodium azide.
Preparation and Storage
SHBG antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year.

Western Blot (WB)

(Western blot analysis of SHBG in human liver tissue lysate with SHBG antibody at (A) 1 and (B) 2 μg/ml.)

Western Blot (WB) (Western blot analysis of SHBG in human liver tissue lysate with SHBG antibody at (A) 1 and (B) 2 μg/ml.)

Immunofluorescence (IF)

(Immunofluorescence of SHBG in mouse liver tissue with SHBG antibody at 20 μg/ml.)

Immunofluorescence (IF) (Immunofluorescence of SHBG in mouse liver tissue with SHBG antibody at 20 μg/ml.)
Related Product Information for anti-SHBG antibody
SHBG is a steroid binding protein that was first described as a plasma protein secreted by the liver and is thought to participate in the regulation of steroid responses. SHBG transports androgens and estrogens in the blood, binding each steroid molecule as a dimer formed from identical or nearly identical monomers (1). Low plasma SHBG levels are associated with obesity, abdominal adiposity, metabolic syndrome, and predict the development of type 2 diabetes (2,3). Polymorphisms in this gene have been associated with polycystic ovary syndrome and type 2 diabetes mellitus (4).

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Predicted: 25; 26; 31; 32; 38; 44 kDa

Observed: 35 kDa
NCBI Official Full Name
sex hormone-binding globulin isoform 1
NCBI Official Synonym Full Names
sex hormone-binding globulin
NCBI Official Symbol
SHBG
NCBI Official Synonym Symbols
ABP; SBP; TEBG
NCBI Protein Information
sex hormone-binding globulin; sex steroid-binding protein; testosterone-binding beta-globulin; testosterone-estrogen-binding globulin; testosterone-estradiol-binding globulin; testis-specific androgen-binding protein
UniProt Protein Name
Sex hormone-binding globulin
UniProt Gene Name
SHBG
UniProt Synonym Gene Names
SHBG; SBP; ABP; TeBG
UniProt Entry Name
SHBG_HUMAN

NCBI Description

This gene encodes a steroid binding protein that was first described as a plasma protein secreted by the liver but is now thought to participate in the regulation of steroid responses. The encoded protein transports androgens and estrogens in the blood, binding each steroid molecule as a dimer formed from identical or nearly identical monomers. Polymorphisms in this gene have been associated with polycystic ovary syndrome and type 2 diabetes mellitus. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014]

Uniprot Description

SHBG: Functions as an androgen transport protein, but may also be involved in receptor mediated processes. Each dimer binds one molecule of steroid. Specific for 5-alpha-dihydrotestosterone, testosterone, and 17-beta-estradiol. Regulates the plasma metabolic clearance rate of steroid hormones by controlling their plasma concentration. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 17p13.1

Cellular Component: extracellular region

Molecular Function: androgen binding

Biological Process: primary spermatocyte growth

Research Articles on SHBG

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Product Notes

The SHBG shbg (Catalog #AAA153715) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The SHBG Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's SHBG can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunofluorescence (IF). LIN54 antibody can be used for detection of LIN54 by Western blot at 1 - 2 mug/ml. For immunofluorescence start at 20 mug/mL. Researchers should empirically determine the suitability of the SHBG shbg for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SHBG, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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