Human keratin 18 ELISA Kit | KRT18 elisa kit

Human cytokeratin 18, CK-18 ELISA Kit

Cat. Num. AAA702127

• Gene Names: KRT18; K18; CYK18
• Reactivity: Human
• Synonyms: keratin 18; Human cytokeratin 18; CK-18 ELISA Kit; CYK18; K18; cell proliferation-inducing protein 46; cytokeratin 18; KRT18 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of human CK-18. No significant cross-reactivity or interference between human CK-18 and analogues was observed.
• Sequence Length: 430

• Samples: Serum, plasma, tissue homogenates, Cell lysates, cell culture supernates
• Assay Type: Sandwich
• Detection Range: 0.625 ng/ml-15 ng/ml.
• Sensitivity: The minimum detectable dose of human CK-18 is typically less than 0.25 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest human CK-18 concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<10%. Three samples of known concentration were tested twenty times on one plate to assess.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<15%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for KRT18 elisa kit

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for CK-18 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells with a Horseradish Peroxidase (HRP) conjugated antibody specific for CK-18. Following a wash to remove any unbound reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CK-18 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 4557888
• NCBI GeneID: 3875
• NCBI Accession #: NP_000215.1
• NCBI GenBank Nucleotide #: NM_000224.2
• UniProt Accession #: P05783; Q53G38; Q5U0N8; Q9BW26
• Molecular Weight: 48,058 Da
• NCBI Official Full Name: keratin, type I cytoskeletal 18
• NCBI Official Synonym Full Names: keratin 18
• NCBI Official Symbol: KRT18
• NCBI Official Synonym Symbols: K18; CYK18
• NCBI Protein Information: keratin, type I cytoskeletal 18; CK-18; keratin-18; cytokeratin 18; cytokeratin-18; cell proliferation-inducing protein 46; cell proliferation-inducing gene 46 protein
• UniProt Protein Name: Keratin, type I cytoskeletal 18
• Protein Family: Keratin
• UniProt Gene Name: KRT18
• UniProt Synonym Gene Names: CYK18; PIG46; CK-18; K18
• UniProt Entry Name: K1C18_HUMAN

NCBI Description

KRT18 encodes the type I intermediate filament chain keratin 18. Keratin 18, together with its filament partner keratin 8, are perhaps the most commonly found members of the intermediate filament gene family. They are expressed in single layer epithelial tissues of the body. Mutations in this gene have been linked to cryptogenic cirrhosis. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

Function: Involved in the uptake of thrombin-antithrombin complexes by hepatic cells When phosphorylated, plays a role in filament reorganization. Involved in the delivery of mutated CFTR to the plasma membrane. Together with KRT8, is involved in interleukin-6 (IL-6)-mediated barrier protection.7 PublicationsManual assertion based on experiment in:Ref.11