Rabbit anti-Human RPLP2 Polyclonal Antibody | anti-RPLP2 antibody

RPLP2, NT (Renal carcinoma antigen NY-REN-44, 60S acidic ribosomal protein P2, RPP2, D11S2243E, RPLP2) (Azide free) (HRP)

Cat. Num. AAA6343637

• Gene Names: RPLP2; P2; LP2; RPP2; D11S2243E
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: RPLP2; Polyclonal Antibody; NT (Renal carcinoma antigen NY-REN-44; 60S acidic ribosomal protein P2; RPP2; D11S2243E; RPLP2) (Azide free) (HRP); Renal carcinoma antigen NY-REN-44; anti-RPLP2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-RPLP2 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: RPLP2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 20~49 amino acids from the N-terminal region of human RPLP2.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(RPLP2 Antibody (N-Term) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the RPLP2 antibody detected the RPLP2 protein (arrow).)

Related Product Information for anti-RPLP2 antibody

RPLP2 consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This protein encodes a ribosomal phosphoprotein that is a component of the 60S subunit. The protein, which is a functional equivalent of the E. coli L7/L12 ribosomal protein, belongs to the L12P family of ribosomal proteins. It plays an important role in the elongation step of protein synthesis. Unlike most ribosomal proteins, which are basic, the encoded protein is acidic. Its C-terminal end is nearly identical to the C-terminal ends of the ribosomal phosphoproteins P0 and P1. The P2 protein can interact with P0 and P1 to form a pentameric complex consisting of P1 and P2 dimers, and a P0 monomer. The protein is located in the cytoplasm. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.

Product Categories/Family for anti-RPLP2 antibody

Antibodies; Cancer Markers

References

Martinez-Azorin, F. FEBS Lett. 582 (20), 3029-3032 (2008). Martinez-Azorin, F. Biochem. J. 413 (3), 527-534 (2008). Sugiyama, N. Mol. Cell Proteomics 6 (6), 1103-1109 (2007).

NCBI and Uniprot Product Information

• NCBI GI #: 4506671
• NCBI GeneID: 6181
• NCBI Accession #: NP_000995
• NCBI GenBank Nucleotide #: NM_001004.3
• UniProt Accession #: P05387; Q6FG96
• Molecular Weight: 14.2 kDa (139aa) confirmed by MALDI-TOF
• NCBI Official Full Name: 60S acidic ribosomal protein P2
• NCBI Official Synonym Full Names: ribosomal protein lateral stalk subunit P2
• NCBI Official Symbol: RPLP2
• NCBI Official Synonym Symbols: P2; LP2; RPP2; D11S2243E
• NCBI Protein Information: 60S acidic ribosomal protein P2
• UniProt Protein Name: 60S acidic ribosomal protein P2
• UniProt Gene Name: RPLP2
• UniProt Synonym Gene Names: D11S2243E; RPP2
• UniProt Entry Name: RLA2_HUMAN

NCBI Description

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal phosphoprotein that is a component of the 60S subunit. The protein, which is a functional equivalent of the E. coli L7/L12 ribosomal protein, belongs to the L12P family of ribosomal proteins. It plays an important role in the elongation step of protein synthesis. Unlike most ribosomal proteins, which are basic, the encoded protein is acidic. Its C-terminal end is nearly identical to the C-terminal ends of the ribosomal phosphoproteins P0 and P1. The P2 protein can interact with P0 and P1 to form a pentameric complex consisting of P1 and P2 dimers, and a P0 monomer. The protein is located in the cytoplasm. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq, Jul 2008]

Uniprot Description

RPLP2: Plays an important role in the elongation step of protein synthesis. Belongs to the ribosomal protein L12P family.

Protein type: Ribosomal; Translation

Chromosomal Location of Human Ortholog: 11p15.5

Cellular Component: focal adhesion; membrane; cytosol

Molecular Function: structural constituent of ribosome; RNA binding

Biological Process: SRP-dependent cotranslational protein targeting to membrane; translational elongation; cellular protein metabolic process; viral reproduction; translation; translational initiation; mRNA catabolic process, nonsense-mediated decay; gene expression; viral transcription; translational termination; viral infectious cycle