Rabbit FASN Antibody | anti-FASN antibody

FASN Antibody

Cat. Num. AAA8500690

• Gene Names: FASN; FAS; OA-519; SDR27X1
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: Affinity purification
• Synonyms: FASN; FASN Antibody; FAS; MGC14367; MGC15706; OA-519; SDR27X1; anti-FASN antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Purity/Purification: Affinity purification
• Sequence Length: 2511

• Applicable Applications for anti-FASN antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB: 1:500-1:2000; IHC: 1:50-1:200
• Immunogen: Recombinant protein of human FASN
• Storage Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Preparation and Storage: Store at-20 degree C or-80 degree C. Avoid freeze/thaw cycles.

Western Blot (WB)

(Western blot analysis of extracts of various cell lines, using FASN antibody.)

Related Product Information for anti-FASN antibody

Fatty acid synthase (FASN) catalyzes the synthesis of long-chain fatty acids from acetyl-CoA and malonyl-CoA. FASN is active as a homodimer with seven different catalytic activities and produces lipids in the liver for export to metabolically active tissues or storage in adipose tissue. In most other human tissues, FASN is minimally expressed since they rely on circulating fatty acids for new structural lipid synthesis (1).Recently, increased expression of FASN has emerged as a phenotype common to most human carcinomas. In breast cancer, immunohistochemical staining showed that the levels of FASN are directly related to the size of breast tumors (2). Studies also showed that FASN is highly expressed in lung and prostate cancers and that FASN expression is an indicator of poor prognosis in breast and prostate cancer (3-5). Furthermore, inhibition of FASN is selectively cytotoxic to human cancer cells (5). Thus, increased interest has focused on FASN as a potential target for the identification and treatment of cancer as well as metabolic syndrome (6, 7).

Product Categories/Family for anti-FASN antibody

Autophagy antibody; Cancer; Cardiovascular; Cell Biology; Epigenetics & Nuclear Signaling; Developmental Biologys; Immunology; Drug Discovery Products; Metabolism; Neuroscience; Signal Transduction; Stem Cells

References

1. Katsurada, A. et al. (1990) Eur J Biochem 190, 427-33. 2. Wells, W.A. et al. (2006) Breast Cancer Res Treat 98, 231-40. 3. Kawamura, T. et al. (2005) Pathobiology 72, 233-240. 4. Shah, U.S. et al. (2006) Hum Pathol 37, 401-409. 5. Kuhajda, F.P. (2000) Nutrition 16, 202-8. 6. Tian, W.X. (2006) Curr Med Chem 13, 967-977. 7. Kusunoki, J. et al. (2006) Endocrine 29, 91-100.

NCBI and Uniprot Product Information

• NCBI GI #: 41872631
• NCBI GeneID: 2194
• NCBI Accession #: NP_004095.4
• NCBI GenBank Nucleotide #: NM_004104.4
• UniProt Accession #: P49327; Q13479; Q16702; Q4LE83; Q6P4U5; Q6SS02; Q969R1; Q96C68; Q96IT0
• Molecular Weight: 272kDa
• NCBI Official Full Name: fatty acid synthase
• NCBI Official Synonym Full Names: fatty acid synthase
• NCBI Official Symbol: FASN
• NCBI Official Synonym Symbols: FAS; OA-519; SDR27X1
• NCBI Protein Information: fatty acid synthase
• UniProt Protein Name: Fatty acid synthase
• Protein Family: Fatty acid synthase
• UniProt Gene Name: FASN
• UniProt Synonym Gene Names: FAS

NCBI Description

The enzyme encoded by this gene is a multifunctional protein. Its main function is to catalyze the synthesis of palmitate from acetyl-CoA and malonyl-CoA, in the presence of NADPH, into long-chain saturated fatty acids. In some cancer cell lines, this protein has been found to be fused with estrogen receptor-alpha (ER-alpha), in which the N-terminus of FAS is fused in-frame with the C-terminus of ER-alpha. [provided by RefSeq, Jul 2008]

Uniprot Description

Fatty acid synthetase catalyzes the formation of long-chain fatty acids from acetyl-CoA, malonyl-CoA and NADPH. This multifunctional protein has 7 catalytic activities as an acyl carrier protein.