Human ULBP1 (UL16-binding protein 1) ELISA Kit | ULBP1 elisa kit

Human ULBP1 (UL16-binding protein 1) ELISA Kit

Cat. Num. AAA7612208

• Gene Names: ULBP1; N2DL-1; RAET1I; NKG2DL1
• Reactivity: Human
• Synonyms: ULBP1 (UL16-binding protein 1); Human ULBP1 (UL16-binding protein 1) ELISA Kit; UL16-binding protein 1; ALCAN-beta; NKG2D ligand 1; N2DL-1; NKG2DL1; Retinoic acid early transcript 1I; N2DL1; RAET1I; ULBP1; ULBP1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of ULBP1. No significant cross-reactivity or interference between ULBP1 and analogues was observed.

• Samples: Serum, plasma, tissue homogenates and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 15.625-1000pg/ml
• Sensitivity: 9.375pg/ml
• Intra-assay Precision: CV<8%
• Inter-assay Precision: CV<10%
• Preparation and Storage: Store at 2-8 degree C for 6 months

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ULBP1 elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Capture antibody was pre-coated onto 96-well plates. And the biotin conjugated antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of target can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 952556270
• NCBI GeneID: 80329
• NCBI Accession #: NP_001304018.1
• NCBI GenBank Nucleotide #: NM_001317089.1
• UniProt Accession #: Q9BZM6; Q5VY81; Q8IZW3; Q8IZX6
• Molecular Weight: 27,997 Da
• NCBI Official Full Name: NKG2D ligand 1 isoform 2
• NCBI Official Synonym Full Names: UL16 binding protein 1
• NCBI Official Symbol: ULBP1
• NCBI Official Synonym Symbols: N2DL-1; RAET1I; NKG2DL1
• NCBI Protein Information: NKG2D ligand 1
• UniProt Protein Name: NKG2D ligand 1
• Protein Family: NKG2D ligand
• UniProt Gene Name: ULBP1
• UniProt Synonym Gene Names: N2DL1; RAET1I; N2DL-1; NKG2DL1

NCBI Description

The protein encoded by this gene is a ligand of natural killer group 2, member D (NKG2D), an immune system-activating receptor on NK cells and T-cells. Binding of the encoded ligand to NKG2D leads to activation of several signal transduction pathways, including those of JAK2, STAT5, ERK and PI3K kinase/Akt. Also, in cytomegalovirus-infected cells, this ligand binds the UL16 glycoprotein and is prevented from activating the immune system. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2015]

Uniprot Description

ULBP1: Ligand for the NKG2D receptor, together with at least ULBP2 and ULBP3. ULBPs activate multiple signaling pathways in primary NK cells, resulting in the production of cytokines and chemokines. Binding of ULBPs ligands to NKG2D induces calcium mobilization and activation of the JAK2, STAT5, ERK and PI3K kinase/Akt signal transduction pathway. In CMV infected cells, interacts with soluble CMV glycoprotein UL16. The interaction with UL16 blocked the interaction with the NKG2D receptor, providing a mechanism by which CMV infected cells might escape the immune system. UL16 also causes ULBP1 to be retained in the ER and cis- Golgi apparatus so that it does not reach the cell surface. Belongs to the MHC class I family.

Protein type: Apoptosis; Cell surface; Membrane protein, GPI anchor

Chromosomal Location of Human Ortholog: 6q25.1

Cellular Component: anchored to plasma membrane; plasma membrane

Molecular Function: antigen binding; natural killer cell lectin-like receptor binding

Biological Process: antigen processing and presentation; natural killer cell activation; natural killer cell mediated cytotoxicity; regulation of immune response