Western Blot (WB)
(Western blot (WB) analysis of MYPT3 polyclonal antibody at 1:500 dilutionLaneA: HEK293T whole cell lysateLaneB: A2780 whole cell lysateLaneC: HCT116 whole cell lysateLaneD: H9C2 whole cell lysateLaneE: CT-26 whole cell lysate)
Rabbit GPR183 Polyclonal Antibody | anti-GPR183 antibody
GPR183 polyclonal antibody
Gene Names
PPP1R16A; MYPT3
Reactivity
Human, Rat, Mouse
Applications
Western Blot, Immunohistochemistry
Purity
The protein was purified from E Coli and the purity is >95% (by SDS-PAGE)
Synonyms
GPR183; Polyclonal Antibody; GPR183 polyclonal antibody; Protein phosphatase 1 regulatory subunit 16A; Myosin phosphatase-targeting subunit 3; PPP1R16A; MYPT3; anti-GPR183 antibody
Host
Rabbit
Reactivity
Human, Rat, Mouse
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
MYPT3 polyclonal antibody detects endogenous levels of MYPT3 protein.
Purity/Purification
The protein was purified from E Coli and the purity is >95% (by SDS-PAGE)
Form/Format
PBS with 0.02% sodium azide, 50% glycerol, pH7.4
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-GPR183 antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
WB: 1:500~1000
Immunogen
Synthetic peptide, corresponding to Human MYPT3
Preparation and Storage
Store at 4 degree C short term. Aliquot and store at -25 degree C long term. Avoid freeze-thaw cycles.
Western Blot (WB)
(Western blot (WB) analysis of MYPT3 polyclonal antibody at 1:500 dilutionLaneA: HEK293T whole cell lysateLaneB: A2780 whole cell lysateLaneC: HCT116 whole cell lysateLaneD: H9C2 whole cell lysateLaneE: CT-26 whole cell lysate)
Western Blot (WB)
(Western blot (WB) analysis of MYPT3 polyclonal antibody at 1:500 dilutionLaneA: HEK293T whole cell lysateLaneB: A2780 whole cell lysateLaneC: HCT116 whole cell lysateLaneD: H9C2 whole cell lysateLaneE: CT-26 whole cell lysate)
Related Product Information for anti-GPR183 antibody
The members of the MYPT family, MYPT1, MYPT2 and MYPT3 are the myosinbinding subunits of myosin phosphatase and an integral component of the myosin protein phosphatase. Myosin phosphatase regulates the interaction of actin and myosin downstream of the guanosine triphosphatase Rho. MYPT1 is localized on stress fibers and is distributed close to the cell membrane and at cell-cell contacts to regulate myosin phosphatase activity. In addition to MYPT1, a novel isoform of MYPT1, MYPT2, also interacts with PPlc. MYPT3, also designated PP16A, inhibits protein phosphatase activity involving phosphorylase, myosin light chain and myosin substrates. It acts as a lipid anchor and binds PP1. MYPT3 localizes primarily to the cell membrane.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
57,811 Da
NCBI Official Full Name
protein phosphatase 1 regulatory subunit 16A
NCBI Official Synonym Full Names
protein phosphatase 1, regulatory subunit 16A
NCBI Official Symbol
PPP1R16A
NCBI Official Synonym Symbols
MYPT3
NCBI Protein Information
protein phosphatase 1 regulatory subunit 16A; myosin phosphatase-targeting subunit 3; protein phosphatase 1, regulatory (inhibitor) subunit 16A; likley ortholog of mouse myosin phosphatase targeting subunit 3
UniProt Protein Name
Protein phosphatase 1 regulatory subunit 16A
Protein Family
UniProt Gene Name
PPP1R16A
UniProt Synonym Gene Names
MYPT3
UniProt Entry Name
PP16A_HUMAN
Uniprot Description
PPP1R16A: Inhibits protein phosphatase 1 activity toward phosphorylase, myosin light chain and myosin substrates.
Protein type: Protein phosphatase, regulatory subunit
Chromosomal Location of Human Ortholog: 8q24.3
Cellular Component: plasma membrane
Molecular Function: protein binding
Research Articles on GPR183
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Product Notes
The GPR183 ppp1r16a (Catalog #AAA3014873) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The GPR183 polyclonal antibody reacts with Human, Rat, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's GPR183 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). WB: 1:500~1000. Researchers should empirically determine the suitability of the GPR183 ppp1r16a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GPR183, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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