Rabbit anti-Human RPL17 Polyclonal Antibody | anti-RPL17 antibody

RPL17, CT (RPL17, 60S ribosomal protein L17, 60S ribosomal protein L23, PD-1) (APC)

Cat. Num. AAA6343381

• Gene Names: RPL17; L17; PD-1; RPL23
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: RPL17; Polyclonal Antibody; CT (RPL17; 60S ribosomal protein L17; 60S ribosomal protein L23; PD-1) (APC); PD-1; anti-RPL17 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-RPL17 antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:50-100; FLISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: RPL17 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 155~184 amino acids from the C-terminal region of human RPL17.
• Conjugate: APC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(RPL17 Antibody (C-term) western blot analysis in A375,HL-60 cell line lysates (35ug/lane).This demonstrates the RPL17 antibody detected the RPL17 protein (arrow).)

Immunohistochemistry (IHC)

(RPL17 Antibody (C-term) IHC analysis in formalin fixed and paraffin embedded colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the RPL17 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Related Product Information for anti-RPL17 antibody

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L22P family of ribosomal proteins. It is located in the cytoplasm. This gene has been referred to as rpL23 because the encoded protein shares amino acid identity with ribosomal protein L23 from Halobacterium marismortui; however, its official symbol is RPL17.

Product Categories/Family for anti-RPL17 antibody

Antibodies; Ribosomal Proteins

References

Andersen, J.S., et al. Nature 433(7021):77-83(2005). Kapp, L.D., et al. Annu. Rev. Biochem. 73, 657-704 (2004). Mazumder, B., et al. Cell 115(2):187-198(2003). Matsuda, A., et al. Oncogene 22(21):3307-3318(2003). Odintsova, T.I., et al. J. Protein Chem. 22(3):249-258(2003). Yoshihama, M., et al. Genome Res. 12(3):379-390(2002).

NCBI and Uniprot Product Information

• NCBI GI #: 4506617
• NCBI GeneID: 6139
• NCBI Accession #: NP_000976.1
• NCBI GenBank Nucleotide #: NM_000985.4
• UniProt Accession #: P18621; Q3KQW2; Q6NZ54; Q7M4M5; B2R4H3; B4E3C2; B5ME31
• Molecular Weight: 21,397 Da
• NCBI Official Full Name: 60S ribosomal protein L17 isoform a
• NCBI Official Synonym Full Names: ribosomal protein L17
• NCBI Official Symbol: RPL17
• NCBI Official Synonym Symbols: L17; PD-1; RPL23
• NCBI Protein Information: 60S ribosomal protein L17; 60S ribosomal protein L23; gene encoding putative NFkB activating protein
• UniProt Protein Name: 60S ribosomal protein L17
• Protein Family: 60S ribosomal protein
• UniProt Gene Name: RPL17
• UniProt Entry Name: RL17_HUMAN

NCBI Description

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L22P family of ribosomal proteins. It is located in the cytoplasm. This gene has been referred to as rpL23 because the encoded protein shares amino acid identity with ribosomal protein L23 from Halobacterium marismortui; however, its official symbol is RPL17. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. Alternative splicing results in multiple transcript variants. Read-through transcription also exists between this gene and the neighboring downstream C18orf32 (chromosome 18 open reading frame 32) gene. [provided by RefSeq, Dec 2010]

Uniprot Description

RPL17: a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L22P family of ribosomal proteins. It is located in the cytoplasm. This gene has been referred to as rpL23 because the encoded protein shares amino acid identity with ribosomal protein L23 from Halobacterium marismortui; however, its official symbol is RPL17. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. Alternative splicing results in multiple transcript variants. Read-through transcription also exists between this gene and the neighboring downstream C18orf32 (chromosome 18 open reading frame 32) gene. [provided by RefSeq, Dec 2010]

Protein type: Ribosomal; Translation

Chromosomal Location of Human Ortholog: 18q21

Cellular Component: nucleus; cytosol

Molecular Function: structural constituent of ribosome

Biological Process: SRP-dependent cotranslational protein targeting to membrane; translational elongation; cellular protein metabolic process; translation; viral reproduction; translational initiation; mRNA catabolic process, nonsense-mediated decay; gene expression; viral transcription; viral infectious cycle; translational termination