Typical Testing Data/Standard Curve (for reference only)
Rat lecithin-cholesterol acyltransferase ELISA Kit | LCAT elisa kit
Rat Lecithin Cholesterol Acyltransferase, LCat ELISA Kit
Reactivity
Rat
Synonyms
lecithin-cholesterol acyltransferase; Rat Lecithin Cholesterol Acyltransferase; LCat ELISA Kit; Rat Lecithin Cholesterol Acyltransferase (LCAT) ELISA Kit; LCAT elisa kit
Reactivity
Rat
Specificity
This assay has high sensitivity and excellent specificity for detection of Rat LCAT. No significant cross-reactivity or interference between Rat LCAT and analogues was observed.
Sequence Length
440
Samples
Milk, Milk Powder, Tissue (Chicken, Pork)
Assay Type
Competitive
Detection Range
0.5 ppb~40.5 ppb
Sensitivity
0.5 ppb
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV%<10%. Three samples of known concentration were tested twenty times on one plate to assess
Inter-assay Precision
Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for LCAT elisa kit
Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Kanamycin antigen. Standards or samples are added to the appropriate microtiter plate wells with Kanamycin specific antibody and Horseradish Peroxidase (HRP) conjugated anti-antibody. The competitive inhibition reaction is launched between pre-coated Kanamycin and Kanamycin in standards or samples with the Kanamycin special antibody. A substrate solution is added to the wells and the color develops in opposite to the amount of Kanamycin in the standards or samples. The color development is stopped and the intensity of the color is measured!!Background/Introduction: Kanamycin is an aminoglycoside antibiotic and is widely used in treating animal diseases. It harms the 8th cranial nerves, causing damages to the vestibular and cochlear. The main manifestations of renal toxicity are the damages of the proximal convoluted tubule, causing protein urine, hematuresis, renal hypofunction, etc. The residues of Kanamycin in animal derived food affect human health, in China and Occident, Kanamycin has been limited to use for its neurotoxicity and nephrotoxicity. This kit is a detection product developed based on ELISA technology, with operation time as short as 50 min and a sensitivity of 0.5 ppb, and linear range from 0.5 ppb?40.5 ppb.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
49,560 Da
NCBI Official Full Name
phosphatidylcholine-sterol acyltransferase
NCBI Official Symbol
LCAT
NCBI Protein Information
phosphatidylcholine-sterol acyltransferase; lecithin-cholesterol acyltransferase; phospholipid-cholesterol acyltransferase
UniProt Protein Name
Phosphatidylcholine-sterol acyltransferase
Protein Family
UniProt Gene Name
LCAT
UniProt Entry Name
LCAT_RABIT
Uniprot Description
Function: Central enzyme in the extracellular metabolism of plasma lipoproteins. Synthesized mainly in the liver and secreted into plasma where it converts cholesterol and phosphatidylcholines (lecithins) to cholesteryl esters and lysophosphatidylcholines on the surface of high and low density lipoproteins (HDLs and LDLs). The cholesterol ester is then transported back to the liver. Also produced in the brain by primary astrocytes, and esterifies free cholesterol on nascent APOE-containing lipoproteins secreted from glia and influences cerebral spinal fluid (CSF) APOE- and APOA1 levels. Together with APOE and the cholesterol transporter ABCA1, plays a key role in the maturation of glial-derived, nascent lipoproteins. Required for remodeling high-density lipoprotein particles into their spherical forms Has a preference for plasma 16:0-18:2 or 18:O-18:2 phosphatidylcholines.
Catalytic activity: Phosphatidylcholine + a sterol = 1-acylglycerophosphocholine + a sterol ester.
Enzyme regulation: APOA1 is the most potent activator in plasma. Also activated by APOE, APOC1 and APOA4
Research Articles on LCAT
Similar Products
Product Notes
The Rat LCAT lcat (Catalog #AAA705092) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA705092 ELISA Kit recognizes Rat LCAT. It is sometimes possible for the material contained within the vial of "lecithin-cholesterol acyltransferase, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
