Mouse anti-Human Vimentin Monoclonal Antibody | anti-VIM antibody

Vimentin (VIM) (AP)

Cat. Num. AAA6134534

• Reactivity: Human
• Applications: ELISA, Immunoprecipitation, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: Vimentin; Monoclonal Antibody; Vimentin (VIM) (AP); FLJ36605; anti-VIM antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2a,k
• Clone Number: 3E9
• Specificity: Recognizes human VIM.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).
• Sequence Length: 1591

• Applicable Applications for anti-VIM antibody: ELISA (EIA), Immunoprecipitation (IP), Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: Partial recombinant corresponding to aa41-140 from VIM (AAH30573) with GST tag. MW of the GST tag alone is 26kD.
• Immunogen Sequence: GSALRPSTSRSLYASSPGGVYATRSSAVRLRSSVPGVRLLQDSVDFSLADAINTEFKNTRTNEKVELQELNDRFANYIDKVRFLEQQNKILLAELEQLKG
• Conjugate: AP
• Preparation and Storage: Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(VIM monoclonal antibody, Western Blot analysis of VIM expression in HeLa.)

Western Blot (WB)

(Western Blot analysis of VIM expression in transfected 293T cell line by VIM monoclonal antibody. Lane 1: VIM transfected lysate (53.7kD). Lane 2: Non-transfected lysate.)

Immunofluorescence (IF)

(Immunofluorescence of monoclonal antibody to VIM on HeLa cell. [antibody concentration 40ug/ml])

Immunoprecipitation (IP)

(Immunoprecipitation of VIM transfected lysate using VIM monoclonal antibody and Protein A Magnetic Bead and immunoblotted with VIM rabbit polyclonal antibody.)

Product Categories/Family for anti-VIM antibody

Antibodies; Extracellular Matrix Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 33989649
• NCBI GeneID: 7431
• NCBI Official Full Name: Homo sapiens vimentin, mRNA
• NCBI Official Synonym Full Names: vimentin
• NCBI Official Symbol: VIM
• NCBI Protein Information: vimentin
• Protein Family: Vimentin

NCBI Description

This gene encodes a type III intermediate filament protein. Intermediate filaments, along with microtubules and actin microfilaments, make up the cytoskeleton. The encoded protein is responsible for maintaining cell shape and integrity of the cytoplasm, and stabilizing cytoskeletal interactions. This protein is involved in neuritogenesis and cholesterol transport and functions as an organizer of a number of other critical proteins involved in cell attachment, migration, and signaling. Bacterial and viral pathogens have been shown to attach to this protein on the host cell surface. Mutations in this gene are associated with congenital cataracts in human patients. [provided by RefSeq, Aug 2017]