Rabbit anti-Human RPA4 Polyclonal Antibody | anti-RPA4 antibody

RPA4, CT (RPA4, Replication protein A 30kD subunit, Replication factor A protein 4) (Biotin)

Cat. Num. AAA6343184

• Gene Names: RPA4; HSU24186
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: RPA4; Polyclonal Antibody; CT (RPA4; Replication protein A 30kD subunit; Replication factor A protein 4) (Biotin); Replication factor A protein 4; anti-RPA4 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-RPA4 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: RPA4 antibody is generated from rabbits immunized with a KLH conjugated synthetic between 233-261 amino acids selected from the C-terminal region of human RPA4.
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(RPA4 Antibody (C-term) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the RPA4 antibody detected the RPA4 protein (arrow).)

Related Product Information for anti-RPA4 antibody

This gene encodes a single-stranded DNA-binding protein that is the 30-kDa subunit of the replication protein A complex. Replication protein A is an essential factor for DNA double-strand break repair and cell cycle checkpoint activation. The encoded protein localizes to DNA repair foci and may be involved in the cellular DNA damage response. This protein may also play a role in inhibiting viral replication.

Product Categories/Family for anti-RPA4 antibody

Antibodies; DNA Replication; Repair

References

Kemp, M.G., et al. J. Biol. Chem. 285(7):4788-4797(2010). Guey, L.T., et al. Eur. Urol. 57(2):283-292(2010). Haring, S.J., et al. Nucleic Acids Res. 38(3):846-858(2010). Shen, M., et al. Environ. Mol. Mutagen. 50(4):285-290(2009). Mason, A.C., et al. J. Biol. Chem. 284(8):5324-5331(2009).

NCBI and Uniprot Product Information

• NCBI GI #: 9558731
• NCBI GeneID: 29935
• NCBI Accession #: NP_037479.1
• NCBI GenBank Nucleotide #: NM_013347.4
• UniProt Accession #: Q13156; Q3SY03
• Molecular Weight: 28,868 Da
• NCBI Official Full Name: replication protein A 30 kDa subunit
• NCBI Official Synonym Full Names: replication protein A4, 30kDa
• NCBI Official Symbol: RPA4
• NCBI Official Synonym Symbols: HSU24186
• NCBI Protein Information: replication protein A 30 kDa subunit; RP-A p30; RF-A protein 4; replication protein A4, 34kDa; replication factor A protein 4; replication protein A complex 34 kd subunit homolog Rpa4
• UniProt Protein Name: Replication protein A 30 kDa subunit
• Protein Family: Replication protein
• UniProt Gene Name: RPA4
• UniProt Synonym Gene Names: RP-A p30
• UniProt Entry Name: RFA4_HUMAN

Uniprot Description

RPA4: functions as component of the alternative replication protein A complex (aRPA). aRPA binds single-stranded DNA and probably plays a role in DNA repair; it does not support chromosomal DNA replication and cell cycle progression through S-phase. In vitro, aRPA cannot promote efficient priming by DNA polymerase alpha but supports DNA polymerase delta synthesis in the presence of PCNA and replication factor C (RFC), the dual incision/excision reaction of nucleotide excision repair and RAD51-dependent strand exchange. Component of the alternative replication protein A complex (aRPA) composed of RPA1, RPA3 and RPA4. Interacts with RPA1 and RPA3. Localizes to DNA repair foci after DNA damage.

Chromosomal Location of Human Ortholog: Xq21.33

Cellular Component: nucleoplasm; DNA replication factor A complex; nucleus

Molecular Function: single-stranded DNA binding

Biological Process: DNA replication initiation; nucleotide-excision repair; DNA damage checkpoint; DNA replication during S phase; mitotic cell cycle; G1/S transition of mitotic cell cycle; DNA recombination