Mouse Toll-like receptor 1 ELISA Kit | TLR1 elisa kit

Mouse Toll-like receptor 1 ELISA Kit

Cat. Num. AAA763099

• Gene Names: TLR1; TIL; CD281; rsc786; TIL. LPRS5
• Reactivity: Mouse
• Synonyms: Toll-like receptor 1; Mouse Toll-like receptor 1 ELISA Kit; TLR1/CD281/CD281/CD281 antigen/DKFZp547I0610/DKFZp564I0682/KIAA0012MGC126311/MGC104956/MGC126312/rsc786/TIL/TIL. LPRS5/Toll/interleukin-1 receptor-like protein/toll-like receptor 1; TLR1 elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of TLR1. No significant cross-reactivity or interference between TLR1 and analogues was observed.
• Sequence Length: 786

• Samples: Serum, plasma, tissue homogenates and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 0.156-10ng/ml
• Sensitivity: 0.094ng/ml
• Intra-assay Precision: CV<8%
• Inter-assay Precision: CV<10%
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for TLR1 elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Capture antibody was pre-coated onto 96-well plates. And the biotin conjugated antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of target can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 41350337
• NCBI GeneID: 7096
• NCBI Accession #: NP_003254.2
• NCBI GenBank Nucleotide #: NM_003263.3
• Molecular Weight: 90,291 Da
• NCBI Official Full Name: toll-like receptor 1
• NCBI Official Synonym Full Names: toll like receptor 1
• NCBI Official Symbol: TLR1
• NCBI Official Synonym Symbols: TIL; CD281; rsc786; TIL. LPRS5
• NCBI Protein Information: toll-like receptor 1
• UniProt Protein Name: Toll-like receptor 1
• Protein Family: Toll-like receptor
• UniProt Gene Name: TLR1
• UniProt Synonym Gene Names: KIAA0012; TIL
• UniProt Entry Name: TLR1_HUMAN

NCBI Description

The protein encoded by this gene is a member of the Toll-like receptor (TLR) family which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. This gene is ubiquitously expressed, and at higher levels than other TLR genes. Different length transcripts presumably resulting from use of alternative polyadenylation site, and/or from alternative splicing, have been noted for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

TLR1: Participates in the innate immune response to microbial agents. Cooperates with TLR2 to mediate the innate immune response to bacterial lipoproteins or lipopeptides. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Binds MYD88 (via TIR domain). Interacts with CNPY3. Interacts (via extracellular domain) with TLR2. Ligand binding induces the formation of a heterodimer with TLR2. Ubiquitous. Highly expressed in spleen, ovary, peripheral blood leukocytes, thymus and small intestine. Belongs to the Toll-like receptor family.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 4p14

Cellular Component: Golgi apparatus; integral to plasma membrane; lipid raft; membrane; phagocytic vesicle membrane; plasma membrane

Molecular Function: protein binding; protein heterodimerization activity; receptor activity; transmembrane receptor activity

Biological Process: detection of triacylated bacterial lipoprotein; immune response; inflammatory response; innate immune response; macrophage activation; MyD88-dependent toll-like receptor signaling pathway; positive regulation of interleukin-6 biosynthetic process; positive regulation of tumor necrosis factor biosynthetic process; regulation of cytokine secretion; signal transduction; toll-like receptor 1 signaling pathway; toll-like receptor 2 signaling pathway; toll-like receptor 4 signaling pathway; toll-like receptor signaling pathway

Disease: Leprosy, Susceptibility To, 5