Rabbit anti-Human RPA1 Polyclonal Antibody | anti-RPA1 antibody

RPA1, CT (RPA1, REPA1, RPA70, Replication protein A 70kD DNA-binding subunit, Replication factor A protein 1, Single-stranded DNA-binding protein) (Biotin)

Cat. Num. AAA6343162

• Gene Names: RPA1; HSSB; RF-A; RP-A; REPA1; RPA70; MST075
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: RPA1; Polyclonal Antibody; CT (RPA1; REPA1; RPA70; Replication protein A 70kD DNA-binding subunit; Replication factor A protein 1; Single-stranded DNA-binding protein) (Biotin); Single-stranded DNA-binding protein; anti-RPA1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-RPA1 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: RPA1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 530-559 amino acids from the C-terminal region of human RPA1.
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(RPA1 Antibody (C-term) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the RPA1 antibody detected the RPA1 protein (arrow).)

Related Product Information for anti-RPA1 antibody

RPA1 plays an essential role in several cellular processes in DNA metabolism including replication, recombination and DNA repair. Binds and subsequently stabilizes single-stranded DNA intermediates and thus prevents complementary DNA from reannealing. Functions as component of the alternative replication protein A complex (aRPA). aRPA binds single-stranded DNA and probably plays a role in DNA repair; it does not support chromosomal DNA replication and cell cycle progression through S-phase. In vitro, aRPA cannot promote efficient priming by DNA polymerase alpha but supports DNA polymerase delta synthesis in the presence of PCNA and replication factor C (RFC), the dual incision/excision reaction of nucleotide excision repair and RAD51-dependent strand exchange.

Product Categories/Family for anti-RPA1 antibody

Antibodies; DNA Replication; Repair

References

Choi, J.H., et al. Proc. Natl. Acad. Sci. U.S.A. 107(31):13660-13665(2010). Locatelli, G.A., et al. Biochem. J. 429(3):573-582(2010). Briggs, F.B., et al. Am. J. Epidemiol. 172(2):217-224(2010). Guillem, V.M., et al. Am. J. Hematol. 85(7):482-486(2010). Oakley, G.G., et al. Front. Biosci. 15, 883-900 (2010).

NCBI and Uniprot Product Information

• NCBI GI #: 4506583
• NCBI GeneID: 6117
• NCBI Accession #: NP_002936.1
• NCBI GenBank Nucleotide #: NM_002945.3
• UniProt Accession #: P27694; Q59ES9; A8K0Y9
• Molecular Weight: 616
• NCBI Official Full Name: replication protein A 70 kDa DNA-binding subunit
• NCBI Official Synonym Full Names: replication protein A1, 70kDa
• NCBI Official Symbol: RPA1
• NCBI Official Synonym Symbols: HSSB; RF-A; RP-A; REPA1; RPA70; MST075
• NCBI Protein Information: replication protein A 70 kDa DNA-binding subunit; MSTP075; RP-A p70; RF-A protein 1; replication factor A protein 1; single-stranded DNA-binding protein
• UniProt Protein Name: Replication protein A 70 kDa DNA-binding subunit
• Protein Family: Replication factor
• UniProt Gene Name: RPA1
• UniProt Synonym Gene Names: REPA1; RPA70; RP-A p70
• UniProt Entry Name: RFA1_HUMAN