anti-Human TIF1 gamma Polyclonal Antibody | anti-TIF1 gamma antibody

Anti-TIF1 gamma Antibody

Cat. Num. AAA178322

• Gene Names: TRIM33; ECTO; PTC7; RFG7; TF1G; TIF1G; TIFGAMMA; TIF1GAMMA
• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry
• Purity: Immunogen Affinity Purified
• Synonyms: TIF1 gamma; Polyclonal Antibody; Anti-TIF1 gamma Antibody; E3 ubiquitin-protein ligase TRIM33; 8030451N04Rik; AI413936; cb1085; DKFZp586K1123; EC 6.3.2.-; Ectodermin; Ectodermin homolog; FLJ11429; FLJ32925; id:ibd2175; MGC136680; mKIAA1113; OTTHUMP00000013662; OTTHUMP00000013663; Protein Rfg7; PTC7; Ret fused gene 7; RET-fused gene 7 protein; RFG7; Rfg7 protein; TF1G; TIF1-gamma; TIF1G; TIF1GAMMA; TIFGAMMA; Transcription intermediary factor 1-gamma; Transcriptional intermediary factor 1 gamma; TRI33_HUMAN; trim33; Tripartite motif containing 33; Tripartite motif containing 33 protein; tripartite motif-containing 33; Tripartite motif-containing protein 33; wu:fc17f10; zgc:136680; tripartite motif containing 33; anti-TIF1 gamma antibody

• Reactivity: Human
• Clonality: Polyclonal
• Purity/Purification: Immunogen Affinity Purified
• Form/Format: Lyophilized. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
• Sequence Length: 1127

• Applicable Applications for anti-TIF1 gamma antibody: Western Blot (WB), Immunohistochemistry (IHC) Paraffin
• Application Notes: Western Blot Concentration: 0.1-0.5ug/ml; Immunohistochemistry (IHC) Paraffin Concentration: 0.5-1ug/ml
• Immunogen: E Coli-derived human TIF1 gamma recombinant protein (Position: M1001-K1127). Human TIF1 gamma shares 96.1% amino acid (aa) sequence identity with mouse TIF1 gamma.
• Ig Type: Rabbit IgG
• Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Preparation and Storage: At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquoted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

Western Blot (WB)

(Anti- TIF1 gamma Picoband antibody, MBS178322, Western blottingAll lanes: Anti TIF1 gamma (MBS178322) at 0.5ug/mlLane 1: 22RV1 Whole Cell Lysate at 40ugLane 2: SW620 Whole Cell Lysate at 40ugPredicted bind size: 150KDObserved bind size: 150KD )

Immunohistochemistry (IHC)

(Anti- TIF1 gamma Picoband antibody, MBS178322, IHC(P)IHC(P): Human Placenta Tissue )

Related Product Information for anti-TIF1 gamma antibody

Description: Rabbit IgG polyclonal antibody for E3 ubiquitin-protein ligase TRIM33(TRIM33) detection. Tested with WB, IHC-P in Human.

Background: Tripartite motif-containing 33 (TRIM33), also known as transcriptional intermediary factor 1 gamma (TIF1-gamma), is a human gene. The TRIM33 gene is mapped to chromosome 1p13 by FISH. The protein encoded by this gene is thought to be a transcriptional corepressor. However, molecules that interact with this protein have not yet been identified. The protein is a member of the tripartite motif family. This motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. Three alternatively spliced transcript variants for this gene have been described; however, the full-length nature of one variant has not been determined.

References

1. "Entrez Gene: TRIM33 tripartite motif-containing 33". 2. He, W., Dorn, D. C., Erdjument-Bromage, H., Tempst, P., Moore, M. A. S., Massague, J. Hematopoiesis controlled by distinct TIF1-gamma and Smad4 branches of the TGF-beta pathway. Cell 125: 929-941, 2006. 3. Venturini, L., You, J., Stadler, M., Galien, R., Lallemand, V., Koken, M. H. M., Mattei, M. G., Ganser, A., Chambon, P., Losson, R., de The, H. TIF1-gamma, a novel member of the transcriptional intermediary factor 1 family. Oncogene 18: 1209-1217, 1999.

NCBI and Uniprot Product Information

• NCBI GI #: 74027249
• NCBI GeneID: 51592
• NCBI Accession #: NP_056990.3
• NCBI GenBank Nucleotide #: NM_015906.3
• UniProt Accession #: Q9UPN9; O95855; Q5TG72; Q5TG73; Q5TG74; Q9C017; Q9UJ79
• Molecular Weight: 120,541 Da
• NCBI Official Full Name: E3 ubiquitin-protein ligase TRIM33 isoform alpha
• NCBI Official Synonym Full Names: tripartite motif containing 33
• NCBI Official Symbol: TRIM33
• NCBI Official Synonym Symbols: ECTO; PTC7; RFG7; TF1G; TIF1G; TIFGAMMA; TIF1GAMMA
• NCBI Protein Information: E3 ubiquitin-protein ligase TRIM33
• UniProt Protein Name: E3 ubiquitin-protein ligase TRIM33
• UniProt Gene Name: TRIM33
• UniProt Synonym Gene Names: KIAA1113; RFG7; TIF1G; Protein Rfg7; TIF1-gamma
• UniProt Entry Name: TRI33_HUMAN

NCBI Description

The protein encoded by this gene is thought to be a transcriptional corepressor. However, molecules that interact with this protein have not yet been identified. The protein is a member of the tripartite motif family. This motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. Three alternatively spliced transcript variants for this gene have been described, however, the full-length nature of one variant has not been determined. [provided by RefSeq, Jul 2008]

Uniprot Description

TIF1G: an atypical protein kinase that functions as a transcriptional repressor. Translocation with RET generates the TRIM33/RET (PTC7) oncogene. The protein is a member of the tripartite motif family. This motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. Two splice variant isoforms have been described.

Protein type: Ubiquitin ligase; EC 6.3.2.-; Ligase; Transcription, coactivator/corepressor; Protein kinase, atypical; Kinase, protein; EC 6.3.2.19; Ubiquitin conjugating system; ATYPICAL group; TIF1 family

Chromosomal Location of Human Ortholog: 1p13.1

Cellular Component: intracellular membrane-bound organelle; nucleoplasm; nucleus

Molecular Function: DNA binding; ligase activity; protein binding; ubiquitin-protein ligase activity; zinc ion binding

Biological Process: negative regulation of BMP signaling pathway; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; protein ubiquitination; regulation of transforming growth factor beta receptor signaling pathway; transcription, DNA-dependent

Disease: Thyroid Carcinoma, Papillary